Detection of Chemically Induced Y-Chromosomal Nondisjunction in Human Spermatozoa

  • Marvin S. Legator
  • Robert W. KappJr.
Part of the Topics in Chemical Mutagenesis book series (TCM, volume 2)


In the area of chemical mutagenesis certain procedures seem to gain considerable popularity even though their relevance to identifying human hazards is questionable or the genetic basis for the tests has yet to be established. Conversely, there are certain procedures where the potential for identifying genetic lesions in relevant systems is considerable, but their utilization is slow to develop. A procedure with great potential for identifying mutagens that induce cytogenetic changes in man is the detection of chemically induced increase in Y chromosomes in human sperm. This procedure, utilizing fluorochromes, may be one of the more important developments in recent years in the field of chemical mutagenesis. The amount of data, however, and presumably the number of investigators evaluating this technique are minimal. In this chapter, we will describe the studies that led to the development of this method, discuss the technique, and present data obtained using this procedure.


Human Spermatozoon Sperm Head Diagnostic Radiation Flagyl Therapy Fluorescent Body 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Marvin S. Legator
    • 1
  • Robert W. KappJr.
    • 2
  1. 1.Division of Environmental Toxicology, Department of Preventive Medicine and Community HealthThe University of Texas Medical BranchGalvestonUSA
  2. 2.East LaboratoryBio/dynamics, Inc.East MillstoneUSA

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