Advertisement

Detection of Chemically Induced Y-Chromosomal Nondisjunction in Human Spermatozoa

  • Marvin S. Legator
  • Robert W. KappJr.
Part of the Topics in Chemical Mutagenesis book series (TCM, volume 2)

Abstract

In the area of chemical mutagenesis certain procedures seem to gain considerable popularity even though their relevance to identifying human hazards is questionable or the genetic basis for the tests has yet to be established. Conversely, there are certain procedures where the potential for identifying genetic lesions in relevant systems is considerable, but their utilization is slow to develop. A procedure with great potential for identifying mutagens that induce cytogenetic changes in man is the detection of chemically induced increase in Y chromosomes in human sperm. This procedure, utilizing fluorochromes, may be one of the more important developments in recent years in the field of chemical mutagenesis. The amount of data, however, and presumably the number of investigators evaluating this technique are minimal. In this chapter, we will describe the studies that led to the development of this method, discuss the technique, and present data obtained using this procedure.

Keywords

Human Spermatozoon Sperm Head Diagnostic Radiation Flagyl Therapy Fluorescent Body 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T. Caspersson, S. Farber, G. E. Foley, J. R. Kudynowski, E. J. Modest, E. Simonsson, U. Wagh, and L. Zech, Distinction between extra G-like chromosomes by quinacrine mustard fluorescence analysis, Exp. Cell Res. 49, 219–222 (1968).PubMedCrossRefGoogle Scholar
  2. 2.
    T. Caspersson, S. Faber, G. E. Foley, J. R. Kudynowski, E. J. Modest, E. Simonsson, U. Wagh, and L. Zech, Chemical differentiation along metaphase chromosomes, Exp. Cell. Res. 49, 219 (1968).PubMedCrossRefGoogle Scholar
  3. 3.
    T. Caspersson and L. Zech, Chromosome identification by fluorescence, in: Medical Genetics (V. A. McKusick and R. Claiborne, eds.), pp. 27–88, H. P. Publishing Co., New York (1973).Google Scholar
  4. 4.
    P. Barlow and D. G. Vosa, The Y chromosome in human spermatozoa, Nature (London) 226, 961–962 (1970).CrossRefGoogle Scholar
  5. 5.
    A. T. Sumner, J. A. Robinson and J. J. Evans, Distinguishing between X, Y and YY-bearing human spermatozoa by fluorescence and DNA contact, Nature New Biol. 22, 231–233 (1971).Google Scholar
  6. 6.
    A. T. Sumner and J. A. Robinson, A difference in dry mass between the heads of X-and Y-bearing human spermatozoa, J. Reprod. Fertil. 48, 9–15 (1976).PubMedCrossRefGoogle Scholar
  7. 7.
    H. Thompson, J. Mecylik, and F. Hecht, Reproduction and meiosis in XYY, Lancet 2, 831 (1967).CrossRefGoogle Scholar
  8. 8.
    R. W. Kapp, Jr., D. J. Picciano, and C. C. Jacobson, Y Chromosomal nondisjunction in dibromochloropropane-exposed workmen, Mutat. Res. 64, 47–51 (1979).PubMedGoogle Scholar
  9. 9.
    R. W. Kapp, Jr. Detection of aneuploidy in human sperm, Environ. Health Perspect. 31, 27–31 (1979).PubMedCrossRefGoogle Scholar
  10. 10.
    R. W. Kapp, Jr., and C. B. Jacobson, Analysis of human spermatozoa for Y chromosomes nondisjunction, Teratog. Carcinog. Mutagen. 1, 193–211 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    OSHA, Occupational exposure to 1,2-dibromo-3-chloropropane. Fed. Regist. 1977, 45536-45549 (1977).Google Scholar
  12. 12.
    R. A. Beatty, F-Bodies as Y chromosome markers in mature human spermheads, Cytogenet. Cell Genet. 18, 33–49 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Marvin S. Legator
    • 1
  • Robert W. KappJr.
    • 2
  1. 1.Division of Environmental Toxicology, Department of Preventive Medicine and Community HealthThe University of Texas Medical BranchGalvestonUSA
  2. 2.East LaboratoryBio/dynamics, Inc.East MillstoneUSA

Personalised recommendations