Detection of Chemically Induced Y-Chromosomal Nondisjunction in Human Spermatozoa
In the area of chemical mutagenesis certain procedures seem to gain considerable popularity even though their relevance to identifying human hazards is questionable or the genetic basis for the tests has yet to be established. Conversely, there are certain procedures where the potential for identifying genetic lesions in relevant systems is considerable, but their utilization is slow to develop. A procedure with great potential for identifying mutagens that induce cytogenetic changes in man is the detection of chemically induced increase in Y chromosomes in human sperm. This procedure, utilizing fluorochromes, may be one of the more important developments in recent years in the field of chemical mutagenesis. The amount of data, however, and presumably the number of investigators evaluating this technique are minimal. In this chapter, we will describe the studies that led to the development of this method, discuss the technique, and present data obtained using this procedure.
KeywordsHuman Spermatozoon Sperm Head Diagnostic Radiation Flagyl Therapy Fluorescent Body
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- 3.T. Caspersson and L. Zech, Chromosome identification by fluorescence, in: Medical Genetics (V. A. McKusick and R. Claiborne, eds.), pp. 27–88, H. P. Publishing Co., New York (1973).Google Scholar
- 5.A. T. Sumner, J. A. Robinson and J. J. Evans, Distinguishing between X, Y and YY-bearing human spermatozoa by fluorescence and DNA contact, Nature New Biol. 22, 231–233 (1971).Google Scholar
- 11.OSHA, Occupational exposure to 1,2-dibromo-3-chloropropane. Fed. Regist. 1977, 45536-45549 (1977).Google Scholar