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Frequency-Analysis of Precursors of Cytotoxic T Lymphocytes in Radiation Chimeras: Enumeration of Antigenspecific CTL-P Restricted to Thymic MHC- and Bone Marrow-MHC-Determinants

  • Klaus Pfizenmaier
  • Hubertus Stockinger
  • Martin Krönke
  • Peter Scheurich
  • Conny Hardt
  • Martin Röllinghoff
  • Hermann Wagner

Abstract

The mechanisms controlling the acquisition of T cell restriction specificity and immunocompetence are, despite of numerous investigations, not well understood. From studies of the CTL-immune responsiveness in thymus- and bone marrow-grafted chimeric mice, it became apparent, that it is the thymus which is crucial not only for the maturation or T cells, but also for the specificity repertoire of the T cells (1,2). From these data it was suggested, that during intra-thymic maturation both mutational events and positive selection mechanisms influence the repertoire such that only T cells restricted to thymic epithelial cell MHC determinants mature and will be exported to the peripheral lymphoid organs (3,4). However, the demonstration of non-thymic MHC restricted CTL in both chimeric (5–7) as well as conventional mice (8–10) are incompatible with models proposing strictly thymus-dependent selection mechanisms. Allo-MHC restricted T cells were found not only within spleen cells, but also within thymocytes of both chimeric (9) and non-chimeric mice (10). Accordingly, both self- and allo-MHC restricted thymocytes mature to immunocompetent T cells.

Keywords

Spleen Cell Mass Culture Chimeric Mouse Thymic Epithelial Cell Peripheral Lymphoid Organ 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Klaus Pfizenmaier
    • 1
  • Hubertus Stockinger
    • 1
  • Martin Krönke
    • 1
  • Peter Scheurich
    • 1
  • Conny Hardt
    • 1
  • Martin Röllinghoff
    • 1
  • Hermann Wagner
    • 1
  1. 1.Institut für Med. MikrobiologieUniversität MainzAugustusplatzGermany

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