A Cell-Free Product Secreted by Ly−2+ Cells can Induce a Molecule Required for Ly2 Suppressor Cell Activity
The generation of suppression to SRBC in vitro involves the interaction of different T cell subsets in a well defined regulatory “feedback” circuit. The cells of this circuit have been identified by the correlation of function with a unique profile of cell surface glycoproteins (differentiation antigens). The induction signal is delivered by an I-J+ Ly1+2− cell to an Ly1+2+ I-J+ acceptor cell (1,2). The interaction between the Ly1 inducer cell and the Ly1,2 transducer cell is antigen-specific and restricted by genes linked to the V-region of the immunoglobulin heavy chain locus. The transducer cell then activates the effector cell of the suppressor circuit. One mechanism by which this takes place is the differentiation of transducer cells into effector cells (3). Other mechanisms may exist. The effector cell is so called because of its ability to suppress Ly1 cells directly, without the need for an Ly1,2 transducer cell (4). These effector cells are capable of suppressing not only helper cells, but also the feedback inducer cells. This results not only in a shutdown of antibody synthesis but abrogates additional T suppressor induction and thereby completing the feedback circuit. The phenotype of the effector cell is an Ly1−2+ and, unlike the other cells of the feedback circuit, it does not bear the I-J subregion gene products (4). This is especially important in light of the fact that the interaction between the suppressor effector cell and its target is partially restricted by genes mapping into this same I-J subregion of the MHC.
KeywordsAntigen Binding Assay Culture Plaque Form Cell Transducer Cell Binding Chain
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