Structural Analysis of Cloned Mouse and Human DNA Sequences Specifying C3, the Third Component of Complement
We wished to produce cloned DNA sequences representing C3, the third component of complement and to perform structural characterization of these for three principal purposes. Firstly, we wanted to predict the primary amino acid sequences of the C3 polypeptide from nucleotide sequences of cloned complementary DNA (cDNA). The C3 protein possesses multiple functions due to its capacity to interact specifically with other complement components (e.g. convertase complexes in the classical and alternative pathway), the control proteins I and H of the alternative pathway of complement activation and with several different types of C3 receptor molecules in the surface membranes of different classes of cells (1–4). Inspite of the important role of C3 in the defence of the human organism against infection and inspite of the fact, that it can be purified in large quantities (5), only very scarce primary amino acid sequence data have been accumulated. The published sequences cover: the C3a anaphylatoxin peptide (6,7), the amino- and carboxy- termini of the mature α, α’ and β subunits (8,9), the aminoterminus of the pro-C3 precursor polypeptide (10) and the part of the C3d peptide fragment, that contains the internal thiolester site (11,12).
KeywordsPrimary Amino Acid Sequence Tamic Acid Southern Blot Experiment eDNA Probe Generate Restriction Enzyme
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