Development of Sensitivity to Catecholamines in Granulosa and Luteal Cells

  • Gunnar Selstam
  • Sheela Rani
  • Knut Nordenstrom
  • Ensio Norjavaara
  • Sten Rosberg
  • Kurt Ahrén
Part of the Biochemical Endocrinology book series (BIOEND, volume 1)


Catecholamines have a wide variety of physiological effects on a number of organ systems, including the nervous system, the circulation and the metabolism. The first indications that catecholamines could influence gonadal function was probably the observation that stress can depress the androgen blood level in the male (Levin et al., 1967) and the observation that the fibromuscular layer surrounding the mature follicle can increase the intrafollicular pressure and possibly promote the ovulatory process (Walles et al., 1976). The role of these effects are, however, still under debate. Catecholamines also have effects on the blood vessels of the gonads resulting in changes in blood flow (e.g. Damber et al., 1982; Selstam, 1975). The arteries are innervated (Burder, 1978), but the role of catecholamines on gonadal blood flow also remains to be investigated.


Granulosa Cell Adenylate Cyclase Corpus Luteum Adenylate Cyclase Activity Luteal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abramowitz, J., Iyengar, R., and Birnbaumer, L., 1982, Guanine nucleotide and magnesium ion regulation of the interaction of gonadotropic and -adrenergic receptors with their hormones: A comparative study using a single membrane system, Endocrinology, 110:336.PubMedCrossRefGoogle Scholar
  2. Adashi, E. Y., and Hsueh, A. J. W., 1981, Stimulation of -adrenergic responsiveness by follicle stimulating hormone in rat granulosa cells in vitro and in vivo, Endocrinology, 108:2170.PubMedCrossRefGoogle Scholar
  3. Ahrén, K., and Selstam, G., 1971, Hormonal regulation of ovarian phosphorylase activity, Acta Physiol. Scand., 82:31.CrossRefGoogle Scholar
  4. Ahrén, K., Norjavaara, E., Rosberg, S., and Selstam, G., Development of prostaglandin F2a-inhibition of epinephrine stimulated cyclic AMP and progesterone production by rat corpora lutea, Manuscript in preparation.Google Scholar
  5. Ahrén, K., Bergh, C., Ekholm, C., Hamberger, L., Hillensjö, T., Khan, I., Nilsson, L., Nordenström, K., Rosberg, S., and Selstam, G., 1979, Development of refractoriness of the ovarian cyclic AMP system to gonadotropins, in: Advances in the Biosciences: Development of Responsiveness to Steroid Hormones, A. M. Kaye and M. Kaye, eds., pp. 319341, Pergamon Press, Oxford.Google Scholar
  6. Bahr, J., Kao, L., and Nalbandov, A. W., 1974, The role of catecholamines and nerves in ovulation, Biol. Reprod., 10:273.PubMedCrossRefGoogle Scholar
  7. Ben-Jonathan, N., Baw, R. H., Laufer, N., Reich, R., Bahr, J. M., and Tsafriri, A., 1982, Norephinephrine in Graafian follicles is depleted by follicle stimulating follicle stimulating hormone, Endocrinology, 110:457.PubMedCrossRefGoogle Scholar
  8. Birnbaumer, L., Yang, P. C, Hunzicker-Dunn, M., Bockaert, J., and Duran, J. M., 1976, Adenylyl cyclase activities in ovarian tissues. I. Homogenization and conditions of assay in Graafian follicles and corpora lutea of rabbits, rats and pigs: Regulation of ATP, and some comparative properties, Endocrinology, 99:163.PubMedCrossRefGoogle Scholar
  9. Burden, H. W., 1978, Ovarian innervation, in: “The Vertebrate Ovary”, R. E. Jones, ed., pp. 615–638, Plenum Press, New York.Google Scholar
  10. Coleman, A. J., Paterson, D. S., and Somerville, A. R., 1979, the -adrenergic receptor of rat corpus luteum membranes, Biochem. Pharmacol., 28:1003.PubMedCrossRefGoogle Scholar
  11. Condon, W. A., and Black, D. L., 1976, Catecholamine induced stimulation of progesterone by the bovine corpus luteum in vitro, Biol. Reprod., 15:573.PubMedCrossRefGoogle Scholar
  12. Damber, J. -E., Lindahl, O., Selstam, G., and Tenland, T., 1982, Testicular blood flow measured with a laser doppler flow meter: Acute effects of catecholamines, Acta. Physiol. Scand., 115:209.PubMedCrossRefGoogle Scholar
  13. Gilman, A. G., 1970, A protein binding assay for adenosine 3’,5’ cyclic monophosphate, Proc. Natl. Acad. Sci. USA, 67:305.PubMedCrossRefGoogle Scholar
  14. Godkin, J. D., Black, D. L., and Dudy, R. T., 1977, Stimulation of cyclic AMP and progesterone synthesis by LH, PGE2 and isoprotereol in bovine corpus luteum in vitro, Biol. Reprod., 17:514.PubMedCrossRefGoogle Scholar
  15. Herlitz, H., Hamberger, L., Rosberg, S., and Ahrén, K., 1974, Cyclic AMP in isolated corpora lutea of the rat: Influences of gonadotropins and prostaglandins, Acta Endocrinol. (Copenh.), 77:737.Google Scholar
  16. Herlitz, H., Koch, Y., and Khan, I., and Ahrén, K., 1976, Effect of follicle stimulating hormone on cyclic AMP levels in young corpora lutea of rat, Eur. J. Obstet. Gyn. Reprod. Biol., 6:175.CrossRefGoogle Scholar
  17. Hunzicker-Dunn, M., 1982, Epinephrine-sensitive adenylyl cyclase activity in rabbit ovarian tissues, Endocrinology, 110:233.PubMedCrossRefGoogle Scholar
  18. Janson, P. -O., LeMaire, W. J., Källfeldt, B., Holmes, P. V., Cajander, S., Bjersing, L., Wigvist, N., and Ahrén, K., 1982, The study of ovulation in the isolated perfused rabbit ovary. I. Methodology and pattern of steroidogenesis, Biol. Reprod., 26:456.PubMedCrossRefGoogle Scholar
  19. Jordan, A. W., 1981, Changes in ovarian -adrenergic receptors during the estrous cycle of the rat, Biol. Reprod., 24:245.PubMedCrossRefGoogle Scholar
  20. Jordan, A. W., Caffrey, J. L., and Niswender, G. D., 1978, Catecholamine-induced stimulation of progesterone and adenosine 3’,5’-monophosphate production by dispersed ovine luteal cells, Endocrinology, 103:385.PubMedCrossRefGoogle Scholar
  21. Kahn, I., and Rosberg, S., 1979, Acute suppression by PGF on LH, epinephrine and fluoride stimulation of adenylate cyclase in rat luteal tissue, J. Cycl. Nuc. Res., 5:55.Google Scholar
  22. Kliachoko, S., and Zor, U., 1981, Increase in catecholaminestimulated cyclic AMP and progesterone synthesis in rat granulosa cells during culture, Mol. Cell. Endocrinol., 23:23.CrossRefGoogle Scholar
  23. Levin, J., Lloyd, C. W., Lobotski, J., and Friedrich, E. H., 1967, The effect of epinephrine on testosterone production, Acta Endocrinol. (Copenh.), 55:187.Google Scholar
  24. Marsh, J. M., 1970, The stimulatory effect of luteinizing hormone on adenyl cyclase in bovine corpus luteum, J. Biol. Chem., 245:1596.PubMedGoogle Scholar
  25. Norjavaara, E., Selstam, G., Damber, J. -E., and Johansson, B. -M., Stimulatory effect of noradrenaline in vivo on the cyclic AMP level in rat corpora lutea. (Manuscript in prep).Google Scholar
  26. Norjavaara, E., Selstam, G., and Ahrén, K., 1982, Catecholamine stimulation of cyclic AMP and progesterone production in rat corpora lutea of different ages, Acta Endocrinol. (Copenh.), 100:613.Google Scholar
  27. Owman, C., and Sjöberg, N. -O., 1966, Adrenergic nerves in the female genital tract of the rabbit with remarks on cholinesterase containing-structures, Z. Zell forsch, 74:182.CrossRefGoogle Scholar
  28. Owman, C., Sjöberg, N. -O., Wallach, E. E., Walles, E., and Wright, K. H., 1979, Neuromuscular mechanisms of ovulation, in: “Human Ovulation: Mechanisms, Prediction, Detection and Induction”, E. S. E. Haez, ed., pp. 57–100, North Holland, Amsterdam.Google Scholar
  29. Rani, S., and Moudgal, N. R., 1977, Examination of the role of FSH in preovulatory events in the hamster, J. Reprod. Fertil., 50:37.PubMedCrossRefGoogle Scholar
  30. Rani, S., Nordenström, K., Norjavaara, E., and Ahrén, K., Development of catecholamine responsiveness in granulosa cells from preovulatory rat follicles -dependence on preovulatory LH surge. (Manuscript in preparation.)Google Scholar
  31. Rosberg, S., Khan, I., Selstam, G., and Ahrén, K., 1981, LH-induced desensitization of adenylate cyclase in membranes of rat corpora lutea, Acta Endocrinol. (Copenh.), suppl. 243, 97:68.Google Scholar
  32. Selstam, G., 1975, Studies on regulatory mechanisms of the cyclic AMP system in the ovary. Thesis, University of Gothemburg, Sweden, p. 15.Google Scholar
  33. Selstam, G., Norjavaara, E., Rosberg, S., Damber, J. -E., and Cajander, S., Studies of the luteinization process: Induction of catecholamine response on adenylate cyclase activity by LH. (Manuscript in preparation.)Google Scholar
  34. Selstam, G., Norjavaara, E., Rosberg, S., Khan, I., Hamberger, B., and Hamberger, L., 1982, Catecholamine content and effects of luteinizing hormone and catecholamines on adenylate cyclase activity in rat corpora lutea on different ages. (Manuscript in preparation.)Google Scholar
  35. Walles, B., Edvinsson, L., Owman, C., Sjoberg, N. -O., and Sporrong, B., 1976, Cholinergic nerves and receptors mediating contraction of the Graafian follicle, Biol. Reprod., 17:423.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Gunnar Selstam
    • 1
  • Sheela Rani
    • 1
  • Knut Nordenstrom
    • 1
  • Ensio Norjavaara
    • 1
  • Sten Rosberg
    • 1
  • Kurt Ahrén
    • 1
  1. 1.Departments of PhysiologyUniversities of Umeåa and GothenburgSweden

Personalised recommendations