Regulation of Steroidogenesis in Isolated Leydig Cells
It is well-known that LH plays an important role in the (acute) regulation of steroidogenesis in Leydig cells. In the last years, it has become clear that in addition to LH, hormones such as prolactin and oestradiol, as well as an intratesticular LHRH-like factor, may also play an important role in the (long-term) regulation of the responsiveness of Leydig cells to LH. The ultimate regulation of the production of biologically active steroids in Leydig cells is the result of interactions of many stimulatory and inhibitory processes, influenced by different hormones and/or factors. Investiga- tion of isolated intact cells under defined conditions enables a dissection of individual regulatory systems. It appears, however, that the quality of isolated cells is not always optimal and that different in vitro conditions may greatly influence the steroid production by Leydig cells.
KeywordsLeydig Cell Steroid Production Relative Specific Activity Steroidogenic Activity General Protein Synthesis
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