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Development and Purification of Calmodulin Andibodies: Use in Elucidating Multiple Functions of the Protein in Eukaryotic Cells

  • Anthony R. Means
  • James G. Chafouleas
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Calmodulin (CaM) is the main intracellular Ca receptor in eukaryotic cells. Some aspects are now considered.— I. CaM antibodies as useful biological probes. II. Purification of CaM. III. Production of antibodies, polyclonal or monoclonal, to native CaM. IV. Development of a radioimmunoassay for CaM, and of a way to immunoprecipitate CaM from a complex mixture of cell proteins.

Keywords

Borate Buffer Booster Injection Ammonium Carbonate Coupling Buffer Cell BioI 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Anthony R. Means
    • 1
  • James G. Chafouleas
    • 1
  1. 1.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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