Specific High-Affinity Membrane Receptors for Biologically Active Phorbol and Ingenol Esters

  • Mohammed Shoyat
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


We have used [20–3H]phorbol-12,13-dibutyrate (PDBu) to study the specific binding of phorbol and ingenol esters and mezerein to a variety of cells in culture and to animal tissues [1]. 3H-PDBu binds to a variety of normal and transformed avian and mammalian cells (mouse, rat, cat, mink, hamster, rabbit, bat, dog, monkey, and human) in a specific, saturable and reversible manner. The specific binding sites are also present in brain, spleen, thymus, lung, skin, kidney, heart, stomach, thigh muscle, liver and intestine in decreasing order. Neither human nor mouse erythrocytes bind detectable amounts of 3H-PDBu. Brain and spleen from all mouse strains bind an exceptionally high amount of PDBu. The binding increases progressively with age up to one month and remains almost the same till old age [2].


Thigh Muscle Sodium Fluoride Specific Binding Site Mouse Erythrocyte Specific Membrane Receptor 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Shoyab, M. & Todaro, G.J. (1980) Nature 288, 451–455.CrossRefGoogle Scholar
  2. 2.
    Shoyab, M., Warren, T.C. & Todaro, G.J. (1981) Carcinogenesis 2, 1273–1276.CrossRefGoogle Scholar
  3. 3.
    Shoyab, M., De Larco, J.E. & Todaro, G.J. (1979) Nature 279, 387–391.CrossRefGoogle Scholar
  4. 4.
    Shoyab, M., Todaro, G.J. & Tallman, J.F. (1982) Cancer Lett. 16, 171-CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Mohammed Shoyat
    • 1
  1. 1.Laboratory of Viral CarcinogenesisNational Cancer InstituteFrederickUSA

Personalised recommendations