Specific High-Affinity Membrane Receptors for Biologically Active Phorbol and Ingenol Esters
We have used [20–3H]phorbol-12,13-dibutyrate (PDBu) to study the specific binding of phorbol and ingenol esters and mezerein to a variety of cells in culture and to animal tissues . 3H-PDBu binds to a variety of normal and transformed avian and mammalian cells (mouse, rat, cat, mink, hamster, rabbit, bat, dog, monkey, and human) in a specific, saturable and reversible manner. The specific binding sites are also present in brain, spleen, thymus, lung, skin, kidney, heart, stomach, thigh muscle, liver and intestine in decreasing order. Neither human nor mouse erythrocytes bind detectable amounts of 3H-PDBu. Brain and spleen from all mouse strains bind an exceptionally high amount of PDBu. The binding increases progressively with age up to one month and remains almost the same till old age .
KeywordsThigh Muscle Sodium Fluoride Specific Binding Site Mouse Erythrocyte Specific Membrane Receptor
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