Abstract
Glycine is an inhibitory neurotransmitter in mammalian spinal cord and other regions of the vertebrate nervous system. Its binding to the post-synaptic glycine receptor produces an inhibitory effect on neural firing which is mediated by an increase in chloride conductance and which can be antagonized by strychnine. High-affinity binding sites for [3H]strychnine in rat spinal cord membranes have been shown; the binding is inhibited by glycine and is thought to to occur at the post-synaptic glycine receptor. We therefore used strychnine as the specific ligand for studies aimed at characterizing this receptor biochemically.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pfeiffer, F. & Betz, H. (198l) Brain Res. 226, 273–279.
Graham, D., Pfeiffer, F. & Betz, H. (1981) Biochem. Biophys. Res. Comm. 102, 1330–1335.
Graham, D., Pfeiffer, F. & Betz, H. (1983) Bur. J. Biochem. in press.
Pfeiffer, F., Graham, D. & Betz, H. (1982) J. Biol. Chem. 257, 9389–9393.
Oakley, B.R., Kirsch, D.R. & Morris, N.R. (1980) Anal. Biochem. 105, 361–363.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Graham, D., Pfeiffer, F., Betz, H. (1984). Characterization and Purification of the Glycine Receptor of Rat Spinal Cord. In: Reid, E., Cook, G.M.W., Morré, D.J. (eds) Investigation of Membrane-Located Receptors. Methodological Surveys in Biochemistry and Analysis, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4631-9_62
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4631-9_62
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4633-3
Online ISBN: 978-1-4684-4631-9
eBook Packages: Springer Book Archive