Characterization and Purification of the Glycine Receptor of Rat Spinal Cord
Glycine is an inhibitory neurotransmitter in mammalian spinal cord and other regions of the vertebrate nervous system. Its binding to the post-synaptic glycine receptor produces an inhibitory effect on neural firing which is mediated by an increase in chloride conductance and which can be antagonized by strychnine. High-affinity binding sites for [3H]strychnine in rat spinal cord membranes have been shown; the binding is inhibited by glycine and is thought to to occur at the post-synaptic glycine receptor. We therefore used strychnine as the specific ligand for studies aimed at characterizing this receptor biochemically.
KeywordsPotassium Phosphate Sodium Dodecyl Sulphate Membrane Material Inhibitory Neurotransmitter Glycine Receptor
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