Advertisement

Characterization and Purification of the Glycine Receptor of Rat Spinal Cord

  • David Graham
  • Friedhelm Pfeiffer
  • Heinrich Betz
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Glycine is an inhibitory neurotransmitter in mammalian spinal cord and other regions of the vertebrate nervous system. Its binding to the post-synaptic glycine receptor produces an inhibitory effect on neural firing which is mediated by an increase in chloride conductance and which can be antagonized by strychnine. High-affinity binding sites for [3H]strychnine in rat spinal cord membranes have been shown; the binding is inhibited by glycine and is thought to to occur at the post-synaptic glycine receptor. We therefore used strychnine as the specific ligand for studies aimed at characterizing this receptor biochemically.

Keywords

Potassium Phosphate Sodium Dodecyl Sulphate Membrane Material Inhibitory Neurotransmitter Glycine Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pfeiffer, F. & Betz, H. (198l) Brain Res. 226, 273–279.CrossRefGoogle Scholar
  2. 2.
    Graham, D., Pfeiffer, F. & Betz, H. (1981) Biochem. Biophys. Res. Comm. 102, 1330–1335.CrossRefGoogle Scholar
  3. 3.
    Graham, D., Pfeiffer, F. & Betz, H. (1983) Bur. J. Biochem. in press.Google Scholar
  4. 4.
    Pfeiffer, F., Graham, D. & Betz, H. (1982) J. Biol. Chem. 257, 9389–9393.Google Scholar
  5. 5.
    Oakley, B.R., Kirsch, D.R. & Morris, N.R. (1980) Anal. Biochem. 105, 361–363.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • David Graham
    • 1
  • Friedhelm Pfeiffer
    • 1
  • Heinrich Betz
    • 1
  1. 1.Department of NeurochemistryMax-Planck-Institute of PsychiatryMartinsriedW. Germany

Personalised recommendations