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Determination of Molecular Weights of Receptors in Solution and in Situ

  • E. A. Barnard
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

The mol. wt. of a receptor or receptor complex is an important parameter but one which has proved difficult to determine reliably. The hydrodynamic methods of protein physical chemistry need important modifications to be applicable to receptors, due to the effects of the receptor-hound detergent in the solutions used. Rigorous methods are available now for this, and should be applied. Subunit analyses can also be used, with certain precautions. Determinations so far made of receptor mol. wts. by reliable methods are reviewed.

In the membrane, the only method available is radiation inactivation. This is best used in a relative mode, since attempts to use it absolutely with respect to the radiation dose applied have been susceptible to considerable errors. Applica-tions of this technique which give useful information on receptors and their multiple binding sites in the membrane are also reviewed.

Keywords

Target Size Electric Organ Sedimentation Equilibrium Partial Specific Volume Molecular Asymmetry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • E. A. Barnard
    • 1
  1. 1.Department of BiochemistryImperial College of Science and TechnologyLondonUK

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