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Affinity Probes for Opioid Receptors in Synaptosomes

  • Ahmet Koman
  • Vera Kolb-Meyers
  • Lars Terenius
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Three main approaches to the problem of achieving specific separation and characterization of membrane vesicles from brain (mainly synaptosomes) from brain are being studied.-
  1. 1.

    Affinity chromatography.

     
  2. 2.

    Specific aggregation using dimeric receptor ligands.

     
  3. 3.

    Flow cytometry using fluorescent ligands.

     

Keywords

Affinity Chromatography Opioid Receptor Membrane Vesicle Opioid Peptide Specific Aggregation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Koman, A. & Terenius, L. (1980) FEBS Lett. 118, 293–295.CrossRefGoogle Scholar
  2. 2.
    Hazum, E., Chang, K.-J., Leighton, H.J., Lever Jr., O.W. & Cuatrecasas, P. (1982) Biochem. Biophys. Res. Comm. 104, 347–353.CrossRefGoogle Scholar
  3. 3.
    Shimohigashi, Y., Costa, T., Matsuura, S., Chen, H.-C. & Rodbard, D. (1982) Mol. Pharmacol. 21, 558–563.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Ahmet Koman
    • 1
  • Vera Kolb-Meyers
    • 2
  • Lars Terenius
    • 1
  1. 1.Department of PharmacologyUppsalaSweden
  2. 2.Department of Chemistry and BiochemistrySouthern Illinois UniversityCarbondaleUSA

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