Allosteric Interactions in Receptor Binding Studies
Receptor binding studies can not only detect the competitive interaction of drugs with the binding site for a radiolabeled drug, but also render it possible to monitor the binding of ligands to secondary sites which are allosterically linked to the primary binding site. These modulatory ligands can produce their effects by interacting with a second site on the receptor protein or on other proteins which interact specifically with the receptor. It is possible to obtain information about receptor-effector coupling mechanisms as well as to detect binding sites for drugs which could have novel pharmacological profiles.
KeywordsAdenylate Cyclase Fixed Concentration Primary Binding Site Receptor Binding Study Allosteric Interaction
Unable to display preview. Download preview PDF.
- 5.Stockton, J.M., Birdsall, N.J.M., Burgen, A.S.V. & Hulme, E.C. (1983) Mol. Pharmacol., in press.Google Scholar
- 6.Roszkowski, A.P. (1961) J. Pharm. Exp. Ther. 132, 156–170.Google Scholar
- 8.Maguire, M.E., van Arsdale, P.M. & Gilman, A.G. (1976) Mol. Pharmacol. 12, 335–339.Google Scholar
- 11.Adams, P.R. (1977) J. Physiol. 268, 291–318.Google Scholar
- 12.Shaker, N., Eldefrawi, A.T., Aguayo, L.G., Warnick, J.E., Albuquerque, E.X. & Eldefrawi, M.E. (1982) J. Pharmacol. Exp. Ther. 220, 172–177.Google Scholar
- 13.Colquhoun, D., Dreyer, F. & Sheridan, R.E. (1979) J. Physiol. 293, 247–284.Google Scholar