Subcellular Fractionation of Insect Muscle
We have attempted to subfractionate insect mucle with a view to achieving isolation of nerve terminals. Such preparations, analogous to synaptosomes from mammalian central nervous tissue, would provide attractive systems in vitro in which to study the biochemical characteristics of the insect neuromuscular transmitters, glutamate and γ-aminobutyrate (GABA). Fractions have been obtained which show some features that could be associated with nerve terminals. The fractions cannot at this stage, however, be considered to constitute enriched preparations of nerve terminals. Nevertheless the fractions show specific transport properties for the neurotransmitter amino acids, and may prove useful in characterizing the neurotransmitter systems at the neuromuscular junction. Moreover our experience with the insect muscle serves to emphasize the problems of extrapolating from well established methodologies for mammalian tissue to their invertebrate counterparts.
KeywordsNerve Terminal Mammalian Tissue Neuromuscular Junction Adenylate Kinase Relative Specific Activity
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