Abstract
We have attempted to subfractionate insect mucle with a view to achieving isolation of nerve terminals. Such preparations, analogous to synaptosomes from mammalian central nervous tissue, would provide attractive systems in vitro in which to study the biochemical characteristics of the insect neuromuscular transmitters, glutamate and γ-aminobutyrate (GABA). Fractions have been obtained which show some features that could be associated with nerve terminals. The fractions cannot at this stage, however, be considered to constitute enriched preparations of nerve terminals. Nevertheless the fractions show specific transport properties for the neurotransmitter amino acids, and may prove useful in characterizing the neurotransmitter systems at the neuromuscular junction. Moreover our experience with the insect muscle serves to emphasize the problems of extrapolating from well established methodologies for mammalian tissue to their invertebrate counterparts.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Gerschenfeld, H.M. (1973) Physiol. Rev. 53, 1–119.
Usherwood, P.N.R. ed. (1975) Insect Muscle, Academic Press, London.
Donnellan, J.F., Jenner, D.W. & Ramsey, A. (1974) Insect Biochem. 24, 243–265.
Hoyle, G. (1953) J. Exp. Biol. 30, 121–135.
Cleworth, J.R., Robinson, N.L. & Usherwood, P.N.R. (1980) Soc. Chem. Ind., London, pp. 279–280.
Trevor, A.J., Gordon, M.A. & Parker, K.K. (1978) Life Sci. 23, 1209–1220.
Arch, H.R.S. & Newsholme, E.W. (1978) Essays in Biochemistry [Acad. Press, London] 14, 82–123.
Rubio, R., Berne, R.M. & Dobson, J.G. (1973) Am. J.Physiol. 225, 938–953.
Gibson, W.B. & Drummond, G.I. (1972) Biochemistry 11, 223–229.
Meiners, B.M., Kehoe, P., Shaner, D.M. & Olsen, R.W. (1979) J. Neurochem. 32, 979–990.
Hosie, R.J.A. (1965) Biochem. J. 96, 404–409.
Dahl, J.L. & Hokin, L.E. (1974) Ann. Rev. Biochem. 43, 327–356.
Sacktor, B. (1953) J. Gen. Physiol. 36, 371–387.
Chesky, J.A. (1975) Insect Biochem. 5, 509–518.
Filbin, M.R., Lunt, G.G. & Donnellan, J.F. (1980) in Receptors for Neurotransmitters, Hormones and Pherohormones in Insects (Sattelle, D.B., Hall, L.M. & Hildebrand, J.G., eds.), Elsevier/North Holland, Amsterdam, pp. 153–160.
Cox, D.W.G. & Bradford, H.F. (1978) in Kainic Acid as a Tool in Neurobiology (McGeer, E.G., ed.), Raven Press, New York, pp. 71–93.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Briley, P.A., Lunt, G.G. (1984). Subcellular Fractionation of Insect Muscle. In: Reid, E., Cook, G.M.W., Morré, D.J. (eds) Investigation of Membrane-Located Receptors. Methodological Surveys in Biochemistry and Analysis, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4631-9_53
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4631-9_53
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4633-3
Online ISBN: 978-1-4684-4631-9
eBook Packages: Springer Book Archive