Solubilization and Characterization of Active Opiate Binding Sites
The initial step leading to many of the pharmacological actions of opiates involves their binding to stereospecific membrane-bound receptors. Extraction from brain membranes of opiate receptors that retain their ability to bind opiates in solution has been successfully achieved in our laboratory. Characterization of the soluble receptors demonstrated their close similarity to their membrane-bound counterparts.
KeywordsOpiate Receptor Brain Membrane Opiate Agonist Unlabelled Ligand Lectin Affinity Chromatography
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