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Solubilization and Characterization of Active Opiate Binding Sites

  • Jacob M. Hiller
  • Eric J. Simon
  • Theresa L. Gioannini
  • Richard D. Howells
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

The initial step leading to many of the pharmacological actions of opiates involves their binding to stereospecific membrane-bound receptors. Extraction from brain membranes of opiate receptors that retain their ability to bind opiates in solution has been successfully achieved in our laboratory. Characterization of the soluble receptors demonstrated their close similarity to their membrane-bound counterparts.

Keywords

Opiate Receptor Brain Membrane Opiate Agonist Unlabelled Ligand Lectin Affinity Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Jacob M. Hiller
    • 1
  • Eric J. Simon
    • 1
  • Theresa L. Gioannini
    • 1
  • Richard D. Howells
    • 1
  1. 1.Departments of Psychiatry and PharmacologyNew York University Medical CenterNew YorkUSA

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