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Affinity Labelling of a Cholinergic Nicotinic Receptor in Cultured Cells

  • P. Leprince
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

The application of the methodology used for investigating the muscle nicotinic receptor to the receptor of nerve origin has led to some confusion. Neural nicotinic receptors are generally believed to be insensitive to snake α-neurotoxins, the usual nicotinic markers [1]. Their sparseness does not allow their study by conventional electrophysiological techniques. Thus, in order to identify a suitable marker for this particular protein, some generally less used approaches had to be considered.

Keywords

Nicotinic Receptor Suitable Marker Strong Inhibitory Action Successive Incubation Cholinergic Nicotinic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Patrick, J. & Stallcup, W.B. (1977) J. Biol. Chem. 252, 8629–8633.Google Scholar
  2. 2.
    Green, L.A. & Tischler, A.S. (1976) Proc. Nat. Acad. Sci. 73, 2424–2428.CrossRefGoogle Scholar
  3. 3.
    Karlin, A. (1977) Methods in Enzymol. 46, 582–590.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • P. Leprince
    • 1
  1. 1.Laboratory of BiochemistryUniversity of LiègeLiègeBelgium

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