Affinity Labelling of a Cholinergic Nicotinic Receptor in Cultured Cells
The application of the methodology used for investigating the muscle nicotinic receptor to the receptor of nerve origin has led to some confusion. Neural nicotinic receptors are generally believed to be insensitive to snake α-neurotoxins, the usual nicotinic markers . Their sparseness does not allow their study by conventional electrophysiological techniques. Thus, in order to identify a suitable marker for this particular protein, some generally less used approaches had to be considered.