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Characterization and Isolation of Brain Dopamine (D2) Receptors

  • Philip G. Strange
  • Patricia A. Frankham
  • Jean M. Hall
  • Susan Lancaster
  • Mark Wheatley
  • Raymond M. Withy
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

1. Methods are described for studying the characteristics of brain D2 dopamine receptors using the ligand-binding technique with [3H]spiperone in the native membrane-bound and detergent-solubilized states.

2. Fractionation of brain membranes by differential cent-rifugation and sucrose density-gradient centrifugation gives a preparation showing up to 6-fold enrichment in D2 receptors over the starting homogenate.

3. D2 receptors can be solubilized from brain membranes using detergents. Methods are described for characterizing the solubilized preparations using electron microscopy (e.m.), ultrafiltration, gel filtration, sucrose density-gradient centrifugation and by the ability of specific ligands to bind to the receptors.

4. Useful solubilized D2 receptor preparations can be obtained using lysophosphatidylcholine or cholate (in the presence of sodium chloride).

5. The lysophosphatidylcholine solubilized preparation binds to concanavalin A — Sepharose, which indicates that the D2 receptor is a glycoprotein.

Keywords

Slope Factor Brain Membrane Microsomal Preparation Serotonergic Receptor Benzethonium Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Philip G. Strange
    • 1
  • Patricia A. Frankham
    • 1
  • Jean M. Hall
    • 1
  • Susan Lancaster
    • 1
  • Mark Wheatley
    • 1
  • Raymond M. Withy
    • 1
  1. 1.Department of Biochemistry, The Medical SchoolQueen’s Medical CentreNottinghamUK

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