Examination of Ligand Occupation and the Permeability Response of the Nicotinic Acetylcholine Receptor on Intact Cells

  • Steven M. Sine
  • Palmer Taylor
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Simultaneous measurements in intact cells of receptor occupation and the change in ion permeability elicited by the ligands enable one to define the linkage between agonist association and activation of the ion channel. Two agonist molecules are required for activation, while a single bound antagonist is sufficient to block the response of the receptor oligomer. The two agonist binding sites on the oligomer do not show precise functional equivalence as might be anticipated from the pentameric arrangement of subunit s of the nicotinic acetylcholine receptor.

Keywords

Permeability Oligomer Choline Sine Acetylcholine 

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Steven M. Sine
    • 1
    • 2
  • Palmer Taylor
    • 1
  1. 1.Division of Pharmacology, M-013 H, Department of MedicineUniversity of California, San DiegoLa JollaUSA
  2. 2.Dept. of Molecular NeurobiologySalk InstituteSan DiegoUSA

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