Liposomes as Vehicles for Inhibitors of Intracytoplasmic Protein Synthesis

  • D. P. McIntosh
  • T. D. Heath
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


Different liposome types vary in their capacity for aqueous phase entrapment, the most efficient being those formed by reverse-phase evaporation ([1]; cf. #C-7, this vol.). ‘REVS’ are prepared by sonicating a mixture of phospholipids, organic solvent and aqueous phase with subsequent removal of solvent by reduced-pressure evaporation. The vesicles so formed can be made more homogeneous in size by extruding through a series of polycarbonate membranes [2]. Vesicles may be separated from unencapsulated material by flotation in a discontinuous dextran gradient with centrifugation. By incorporating modified phosphatidylethanolamine in the membrane, sites are provided for the covalent attachment of IgG molecules for targeting purposes.


Diphtheria Toxin Senior Editor Crude Nuclear Fraction Dextran Gradient Double Labelling Procedure 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • D. P. McIntosh
    • 1
  • T. D. Heath
    • 2
  1. 1.Chester Beatty Research Institute, Institute of Cancer Research: RoyalCancer HospitalLondonUK
  2. 2.Cancer Research Institute, School of MedicineUniversity of CaliforniaSan FranciscoUSA

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