Liposomes as Vehicles for Inhibitors of Intracytoplasmic Protein Synthesis
Different liposome types vary in their capacity for aqueous phase entrapment, the most efficient being those formed by reverse-phase evaporation (; cf. #C-7, this vol.). ‘REVS’ are prepared by sonicating a mixture of phospholipids, organic solvent and aqueous phase with subsequent removal of solvent by reduced-pressure evaporation. The vesicles so formed can be made more homogeneous in size by extruding through a series of polycarbonate membranes . Vesicles may be separated from unencapsulated material by flotation in a discontinuous dextran gradient with centrifugation. By incorporating modified phosphatidylethanolamine in the membrane, sites are provided for the covalent attachment of IgG molecules for targeting purposes.
KeywordsDiphtheria Toxin Senior Editor Crude Nuclear Fraction Dextran Gradient Double Labelling Procedure
Unable to display preview. Download preview PDF.
- 3.Stirpe, F., Olsnes, S. & Pihl, A. (1980) J. Biol. Chem. 255, 6947–6953.Google Scholar