Antibody-Targeted Liposomes: Specific Cell Binding and Cytotoxicity
In relation to delivering anti-tumour agents, the interaction of antibody-conjugated liposomes with various cell types in vitro has been studied. Targeted liposomes bearing monoclonal anti H2Kk antibody bind in 6–40 fold greater amounts to cells expressing H2Kk than liposomes bearing anti sheep erythrocyte antibody (non-targeted). The difference between targeted and non-targeted liposomes is increased by incubation in serum. Targeted binding is blocked by soluble anti H2Kk, and is partially inhibited by azide and 2-deoxy-d-glucose.