Preparation of Immunoliposomes
Liposomes have been extensively tested as carriers of drugs, enzymes and other biologically important molecules . One of the major problems of using liposomes as carrier systems is their non-specificity of delivery. In our laboratory, we have attempted to solve this problem by covalently attaching target-specific antibody to liposomes. In the method developed, the antibody is first made amphipathic by the covalent attachment of palmitic acid. The derivatized antibody is then incorporated into liposomal bilayers using a detergentdialysis or modified ‘REV method . We have termed these antibodycarrying liposomes ‘immunoliposomes’. Using a monoclonal antibody (11–4.1) against the murine major histocompatibility antigen, H-2Kk, we have shown that these immunoliposomes bind specifically to target cells carrying the proper cell-surface antigens [3, 4].
KeywordsPalmitic Acid Antibody Molecule Liposomal Bilayer Moloney Leukaemia Virus Palmitoyl Chain
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