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Liposomes Targeted to Cellular Receptors

  • Margaret M. Jonah
  • Yueh-Erh Rahman
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Liposome uptake by cells is influenced by size and charge and by the content of specific glycolipids recognizable by the receptors. Brief descriptions are given of the preparation of liposomes of different types, and of drug encapsulation in the aqueous or lipid phase. Liver parenchymal cells, separated with a Percoll gradient after intravenous liposome injection, take up small unilamellar liposomes (0.06–0.08 μm diam.) preferentially vs. large multilamellar liposomes. Liposomes containing asialo- but not sialo-gangliosides are taken up in vitro by parenchymal but not Kupffer cells; for galactolipid liposomes, parenchymal cells have higher affinity and Kupffer-cell uptake is rather unspecific. In mice, further exemplifying effective targeting to liver parenchymal cells, unilamellar galactolipid-liposomes surpass those without galactolipid in delivering the metal chelator, desferrioxamine, which removes iron located in parenchymal cells.

Unilamellar surpassed multilamellar liposomes in delivering methotrexate to ascites hepatoma 129 cells in mice. In culture these cells showed some preference for galactolipid-liposomes containing methotrexate if encapsulation was in the aqueous phase but not if in the lipid bilayers. Galactolipid-liposomes hardly improved the survival of hepatoma-bearing mice given encapsulated methotrexate. Already it appears that liposomes varying in size and possessing suitable recognition groups on their surfaces are promising for targeting liposome-encapsulated drugs to specific cellular receptors.

Keywords

Parenchymal Cell Kupffer Cell Liposomal Membrane Liver Parenchymal Cell Muramyl Dipeptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Margaret M. Jonah
    • 1
  • Yueh-Erh Rahman
    • 1
  1. 1.Division of Biological and Medical ResearchArgonne National LaboratoryArgonneUSA

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