Affinity Labelling Techniques for Low-Abundance Membrane Components: Peptide Hormone Receptors and the D-Glucose Transporter

  • Jeffrey E. Pessin
  • Joan Massague
  • Michael P. Czech
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


The requirements and effectiveness of affinity labelling are considered in respect of gaining detailed structural information as exemplified by two systems in the field of hormone-activated trans-membrane signalling. Covalent cross-linking of a radioactive ligand was the approach used in peptide hormone receptor studies; structural results are presented for the insulin receptor, which appears to be a disulphide-linked structure with two pairs of subunits. Photoaffinity labelling was the approach used for the D-glucose transporter, an effector for the insulin receptor response; the nature and subcellular location of this transporter are considered.


Insulin Receptor Chicken Embryo Fibroblast Photoaffinity Labelling Detailed Structural Information Affinity Labelling 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Jeffrey E. Pessin
    • 1
  • Joan Massague
    • 1
  • Michael P. Czech
    • 1
  1. 1.Department of BiochemistryUniversity of Massachusetts Medical SchoolWorcesterUSA

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