Binding Sites for Pituitary Growth Hormone and Prolactin in the Liver and Mammary Gland of the Pregnant Rabbit

  • Michael Wallis
  • Hilary F. Cadman
  • Cathryn F. Learoyd
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


The use of 125I-labelled growth hormone (GH) and prolactin to study ‘receptor’ in membrane preparations from rabbit liver and mammary gland is described. Different methods for preparing labelled hormone have been compared, and receptors in a crude microsomal fraction, purified plasma membranes and after solubilization with Triton-X-100 have been studied. The importance of species specificity in the source of hormones is considered. The ability of receptors from rabbit mammary gland to bind various GHs and prolactins accords well with a role for these receptors as mediators of the actions of lactogenic hormones. Binding of GHs and prolactins to liver ‘receptors’, on the other hand, bears little relationship to the grovrth-promoting activity of these various hormones. In an attempt to investigate further the differences between mammary-gland and liver receptors for human GH, the ability of a series of monoclonal antibodies to human GH to inhibit binding has been investigated. It was found that some of these antibodies inhibited receptor binding v/hereas others had little effect, and that the results were similar whether the receptors were obtained from mammary gland or liver.


Growth Hormone Mammary Gland Human Growth Hormone Rabbit Liver Bovine Growth Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wallis, M. (1978) in Biochemistry of Amino Acids, Peptides and Proteins, Vol. 5 (Weinstein, B., ed.), Dekker, New York, pp. 213–320.Google Scholar
  2. 2.
    Lewis, U.J., Singh, R.N.P., Tutwiler, G.F., Sigel, M.B., VanderLaan, E.F. & VanderLaan, W.P. (1980) Recent Prog. Hormone Res.36, 477–508.Google Scholar
  3. 3.
    Wallis, M. (1981) J. Mol. Evolution16, 10–18.CrossRefGoogle Scholar
  4. 4.
    Fraker, P.J. & Speck, J.C. (1978) Biochem. Biophys. Res. Commun.80, 849–857.CrossRefGoogle Scholar
  5. 5.
    Hunter, W.M. & Greenwood, F.C. (1962) Nature194, 495–496.CrossRefGoogle Scholar
  6. 6.
    Roth, J. (1975) Meths. Enzymol.37, 223–233.CrossRefGoogle Scholar
  7. 7.
    Wallis, M., Daniels, M. & Ellis, S.E. (1980) Biochem. J.189, 605–614.Google Scholar
  8. 8.
    Thorell, J.I. & Johansson, B.G. (1971) Biochim. Biophys. Acta251, 363–369.CrossRefGoogle Scholar
  9. 9.
    Tsushima, T. & Friesen, H.G. (1973) J. Clin. Endocr. Metab.37, 334–337.CrossRefGoogle Scholar
  10. 10.
    Neville, D.M. (1968) Biochim. Biophys. Acta154, 540–552.CrossRefGoogle Scholar
  11. 11.
    Goldstein, S. & Blecher, M. (1976) in Methods in Receptor Research, Part I (Blecher, M., ed.), Dekker, New York, pp. 119–142.Google Scholar
  12. 12.
    Cadman, H.F. & Wallis, M. (1981) Biochem. J.198, 605–614.Google Scholar
  13. 13.
    Lesniak, M.A., Gorden, P., Roth, J. & Gavin, J.R. (1974) J. Biol. Chem.249, 1661–1667.Google Scholar
  14. 14.
    Shiu, R.P.C., Kelly, P.A. & Friesen, H.G. (1973) Science180, 968–971.CrossRefGoogle Scholar
  15. 15.
    Shiu, R.P.C. & Friesen, H.G. (1976) Science192, 259–261.CrossRefGoogle Scholar
  16. 16.
    Wallis, M. (1980) in Cellular Receptors for Hormone s and Neurotransmitters (Schulster, D. & Levitski, A., eds.), Wiley, Chichester, pp. 163–183.Google Scholar
  17. 17.
    Herington, A.C. & Veith, N.M. (1977) Endocrinology101, 984–987.CrossRefGoogle Scholar
  18. 18.
    Waters, M.J. & Friesen, H.G. (1979) J. Biol Chem.254, 6815–6825.Google Scholar
  19. 19.
    Tsushima, T., Sasaki, N., Imai, Y., Matsuzaki, F. & Friesen, H.G. (1980) Biochem. J.187, 479–492.Google Scholar
  20. 20.
    Wallis, M. & Cadman, H.F. (1981) Biochem. Soc. Trans.9, 33–35.Google Scholar
  21. 21.
    Posner, B.I., Kelly, P.A., Shiu, R.P.C. & Friesen, H.G. (1974) Endocrinology95, 521–531.CrossRefGoogle Scholar
  22. 22.
    Hughes, J.P. (1979) Endocrinology105, 414–420.CrossRefGoogle Scholar
  23. 23.
    Cadman, H.F., Wallis, M. & Ivanyi, J. (1982) FEBS Lett. 137, 149–152.CrossRefGoogle Scholar
  24. 24.
    Ivanyi, J. & Davies, P. (1980) Moi. Immunol. 17, 287–290.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Michael Wallis
    • 1
  • Hilary F. Cadman
    • 1
  • Cathryn F. Learoyd
    • 1
  1. 1.Biochemistry Laboratory, School of Biological SciencesUniversity of SussexFalmer, BrightonUK

Personalised recommendations