Advertisement

Synaptosomes and Synaptic Membranes: Isolation and Morphology

  • M. Breen
  • H. G. Weinstein
  • P. A. Knepper
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

The isolation of synaptosomes by conventional sucrose densitygradient procedures [l, 2] requires several hours. Several more hours are needed to incubate the synaptosomes prior to being placed in the fixative solution for electron-microscopic (e.m.) studies [3]. Hence it is difficult to complete the process in one working day. Moreover, the iodonitrotetrazolium (1NT) used to increase the buoyant density of mitochondria and so facilitate their removal interferes with the ensuing chemical analyses on the synaptosomes and their membranes. For the above reasons, the Dodd modification of the sucrose density-gradient procedure [4] was tried in which an angle-head rotor was substituted for the swinging-bucket rotor, INT was omitted and discontinuous sucrose gradients were used, enabling the entire procedure to be accomplished in one day.

Keywords

Cholera Toxin Opiate Receptor Synaptic Membrane Disodium EDTA Discontinuous Sucrose Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cotman, C.W., Banker, G., Churchill, L. & Taylor, D. (1974) J. Cell Biol. 63, 441–455.CrossRefGoogle Scholar
  2. 2.
    Kelly, P.T. & Cotman, C.W. (1981) Brain Res. 206, 251–271.CrossRefGoogle Scholar
  3. 3.
    Reynolds, E.A. (1963) J. Cell Biol. 17, 208–211.CrossRefGoogle Scholar
  4. 4.
    Dodd, P.R., Hardy, A.E., Oakley, J.A., Edwardson, J.A., Perry, E.K. & Delaunoy, J.P. (1981) Brain Res. 226, 1971–1975.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • M. Breen
    • 1
  • H. G. Weinstein
    • 1
  • P. A. Knepper
    • 2
  1. 1.Research-in-Aging LaboratoryV.A. Medical CenterNorth ChicagoUSA
  2. 2.Laboratory for Oculo-Cerebrospinal InvestigationChildren’s Memorial HospitalChicagoUSA

Personalised recommendations