Synaptosomes and Synaptic Membranes: Isolation and Morphology
The isolation of synaptosomes by conventional sucrose densitygradient procedures [l, 2] requires several hours. Several more hours are needed to incubate the synaptosomes prior to being placed in the fixative solution for electron-microscopic (e.m.) studies . Hence it is difficult to complete the process in one working day. Moreover, the iodonitrotetrazolium (1NT) used to increase the buoyant density of mitochondria and so facilitate their removal interferes with the ensuing chemical analyses on the synaptosomes and their membranes. For the above reasons, the Dodd modification of the sucrose density-gradient procedure  was tried in which an angle-head rotor was substituted for the swinging-bucket rotor, INT was omitted and discontinuous sucrose gradients were used, enabling the entire procedure to be accomplished in one day.
KeywordsCholera Toxin Opiate Receptor Synaptic Membrane Disodium EDTA Discontinuous Sucrose Gradient
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