Insulin Internalization as Studied by Subcellular Fractionation and Electron-Microscopic Radioautography

  • J. J. M. Bergeron
  • Masood N. Khan
  • Barry I. Posner
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


The techniques of subcellular fractionation and e.m. radioautography have defined novel intracellular components which participate in receptor-mediated endocytosis [1, 2]. These components have been interpreted as vesicles accumulating within the trans* Golgi apparatus (G.a.). The vesicles are highly enriched in receptors for insulin and prolactin; during receptor-mediated insulin internalization the vesicles concentrate reasonably intact insulin prior to donation of hormone to a catabolic compartment (probably the secondary lysosome).


Latent Image Vesicular Structure Secondary Lysosome Lysosomal Fraction Senior Editor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bergeron, J.J.M. & Posner, B.I. (1981) in Receptor-mediated Binding and Internalization of Toxins and Hormones (Middlebrook, J.L. & Kohn, L.D., eds.), Academic Press, New York, pp. 197–215.CrossRefGoogle Scholar
  2. 2.
    Posner, B.I., Bergeron, J.J.M., Josefsberg, Z., Khan, M.N., Khan, R.J., Patel, B.A., Skistrom, R.A. & Verma, A.K. (1981) Rec. Prog. Horm. Res. 37, 539–582.Google Scholar
  3. 3.
    Posner, B.I., Patel, B., Verma, A.K. & Bergeron, J.J.M. (1980) J. Biol. Chem. 255, 735–741.Google Scholar
  4. 4.
    Bergeron, J.J.M., Posner, B.I., Josefsberg, Z. & Sikstrom, R. (1978) J. Biol. Chem. 253, 4058–4066.Google Scholar
  5. 5.
    Khan, N.M., Posner, B.I., Khan, R.J. & Bergeron, J.J.M. (1982) J. Biol. Chem. 257, 5969–5976.Google Scholar
  6. 6.
    Posner, B.I., Patel, B.A., Khan, R.J. & Bergeron, J.J.M. (1982) J. Biol. Chem. 257, 5789–5799.Google Scholar
  7. 7.
    Bergeron, J.J.M., Sikstrom, R., Hand, A.R. & Posner, B.I. (1979) J. Cell Biol. 80, 427–443.CrossRefGoogle Scholar
  8. 8.
    Bergeron, J.J.M., Rachubinski, R.A., Sikstrom, R.A., Posner, B.I. & Paiement, J. (1982) J. Cell Biol. 92, 139–146.CrossRefGoogle Scholar
  9. 9.
    Kopriwa, B.M. (1975) Histochemistry 44, 201–224.CrossRefGoogle Scholar
  10. 10.
    Howell, K.E. & Palade, G.E. (1982) J. Cell Biol. 92, 833–845.CrossRefGoogle Scholar
  11. 11.
    Kasuga, M., Fujita-Yamaguchi, Y., Blithe, D.L. & Khan, C.R. (1983) Proc. Nat. Acad. Sci. 80, 2137–2141.CrossRefGoogle Scholar
  12. 12.
    Posner, B.I., Verma, A.K., Patel, B.A. & Bergeron, J.J.M. (1982) J. Cell Biol. 93, 560–567.- Not cited above; hormone loci.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • J. J. M. Bergeron
    • 1
  • Masood N. Khan
    • 1
  • Barry I. Posner
    • 1
  1. 1.Departments of Anatomy and MedicineMcGill UniversityMontrealCanada

Personalised recommendations