Isolated Adenylate Cyclase and β-Adrenergic Receptor
β-Receptor-sensitive adenylate cyclase can be solubilized in various detergents and partially purified. The partially purified enzyme and the partially purified receptor can be re-incorporated into phospholipid vesicles. The re-constituted β-adrenergic receptor is capable of binding 125I-cyanopindolol, where the affinity for the ligand depends on the phospholipid composition of the vesicle. The hormone sensitivity of the adenylate cyclase is only partially recovered in a full re-constitution assay, most probably because of incomplete functional re-constitution. Achievement of functional re-constitution is the aim of attempts now being made to overcome particular difficulties . We have, however, obtained high-yield re-constitution of the β-receptor with its GTP regulatory unit, and can measure kinetic parameters of the re-constituted system.