Advertisement

Strategy in Receptor Binding Studies

  • N. J. M. Birdsall
  • E. C. Hulme
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

In receptor binding studies, it is important to understand the scope and limitations of both the technique and the results obtained by its application. This article considers some of the methods for optimization of the binding assay technique, for visualization and analysis of binding data, and for investigation of the complexities of the binding of ligands to receptors.

Keywords

Specific Binding Receptor Site Competition Experiment Binding Data Membrane Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Rotter, A., Birdsall, N.J.M., Burgen, A.S.V., Field, P.M., Hulme, E.C. & Raisman, G. (1979) Brain Res. Rev. 1, 141–166.CrossRefGoogle Scholar
  2. 2.
    Wamsley, J.K., Zarbin, M.A., Birdsall, N.J.M. & Kuhar, M.J. (1980) Brain Res. 200, 1–12.CrossRefGoogle Scholar
  3. 3.
    Zarbin, M.A., Wamsley, J.K. & Kuhar, M.J. (1982) J. Neurosci. 2, 934–941.Google Scholar
  4. 4.
    Hulme, E.C., Birdsall, N.J.M., Burgen, A.S.V. & Mehta, P. (1978) Mol. Pharmacol. 14, 737–750.Google Scholar
  5. 5.
    Kent, R.S., DeLean, A. & Lefkowitz, R.J. (1980) Mol. Pharmacol. 17, 14–23.Google Scholar
  6. 6.
    Thakur, A.K., Munson, P.J., Hunston, D.L. & Rodbard, D. (1980) Anal. Biochem. 103, 240–254.CrossRefGoogle Scholar
  7. 7.
    Tobler, H.-J. & Engel, G. (1983) Naunyn Schmiedeberg’s Arch. Pharmacol., in press.Google Scholar
  8. 8.
    Cuatrecasas, P. & Hollenberg, M.D. (1976) Adv. Protein Chem. 30, 252–450.Google Scholar
  9. 9.
    Birdsall, N.J.M. (1983) in Neurotransmitter, Drugs and Disease (Webster, R.A. & Jordan, C.C., eds.), Blackwell, Oxford, in press.Google Scholar
  10. 10.
    DeLean, A., Hancock, A.A. & Lefkowitz, R.J. (1982) Mol. Pharmacol. 21, 5-l6.Google Scholar
  11. 11.
    Birdsall, N.J.M. & Hulme, E.C. (1976) J. Neurochem. 27, 7-l6.CrossRefGoogle Scholar
  12. 12.
    Birdsall, N.J.M., Burgen, A.S.V. & Hulme, E.C. (1978) Mol. Pharmacol. 14, 725–736.Google Scholar
  13. 13.
    Stockton, J.M., Birdsall, N.J.M., Burgen, A.S.V. & Hulme, E.C. (1983) Mol. Pharmacol., 23, 551–557.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • N. J. M. Birdsall
    • 1
  • E. C. Hulme
    • 1
  1. 1.Division of Molecular PharmacologyNational Institute for Medical ResearchMill Hill, LondonUK

Personalised recommendations