Advertisement

Subcellular Strategies for Tracing Receptor-Mediated Ligand Uptake Pathways in Liver

  • W. H. Evans
  • N. Flint
  • N. Hadjiivanova
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Receptor-mediated uptake by liver of asialoglycoproteins, polypeptide hormones and a β-adrenergic antagonist was followed by analytical centrifugation in sucrose gradients of tissue homogenates prepared at various intervals after organ perfusion. All ligands were transferred from the plasma membrane to a vesicular fraction of low density. Problems associated with the preparation of the complex low-density membrane fractions are described, and a preliminary description of their properties is provided.

Keywords

Liver Homogenate Sucrose Gradient Dounce Homogenizer Polypeptide Hormone Analytical Centrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Orlans, E., Peppard, J. Fry, J.R., Hinton, R.H. & Mullock, B.M. (1980) J. Exp. Med. 150, 1577–1581 (and see #B-8, this vol.).Google Scholar
  2. 2.
    Regoeczi, E., Chindemi, P.A., Debanne, M.T. & Charlwood, P.A. (1982) Proc. Nat. Acad. Sci. 79, 2226–2230.CrossRefGoogle Scholar
  3. 3.
    Pastan, I.H. & Willingham, M.C. (1981) Science 214, 504–509.CrossRefGoogle Scholar
  4. 4.
    Farquhar, M.G. (1983) in Membrane Recycling — Ciba Found. Symp. 32 [Pitman, London], 157–174.Google Scholar
  5. 5.
    Fraker, P.J. & Speck, J.C. (1978) Biochem. Biophys. Res. Comm. 80, 849–857.CrossRefGoogle Scholar
  6. 6.
    Engel, G., Hoyer, D., Berthold, R. & Wagner, D. (1981) Arch. Pharmacol. 317, 277–285.CrossRefGoogle Scholar
  7. 7.
    De Duve, C. (1971) J. Cell Biol. 50, 20D–54D.CrossRefGoogle Scholar
  8. 8.
    Beaufay, H. & Amar-Costesec, A. (1976) in Methods in Membrane Biology (Korn, E.D., ed.), Plenum, New York, pp. 1–100.Google Scholar
  9. 9.
    Smith, G.D. & Peters, T.J. (1980) Eur. J. Biochem. 104, 305–311.CrossRefGoogle Scholar
  10. 10.
    Ashwell, G. & Harford, J. (1982) Ann. Rev. Biochem. 51, 531–554.CrossRefGoogle Scholar
  11. 11.
    Smith, G.D. & Peters, T.J. (1982) Biochim. Biophys. Acta 716, 24–30.CrossRefGoogle Scholar
  12. 12.
    Evans, W.H., Flint, N., Debanne, M.T. & Regoeczi, E. (1983) in Structural Carbohydrates of the Liver (Reutter, W., ed.), MTP Press, Lancaster, in press.Google Scholar
  13. 13.
    Smith, G.D., Evans, W.H. & Peters, T.J. (1980) FEBS Lett. 120, 104–106.CrossRefGoogle Scholar
  14. 14.
    Smith, G.D., Flint, N., Evans, W.H. & Peters, T.J. (1981) Biosc. Repts. 1, 921–926.CrossRefGoogle Scholar
  15. 15.
    Schemelck, P.-H. & Hanoune, J. (1980) Mol. Cell. Biochem. 33, 35–48.Google Scholar
  16. 16.
    Debanne, M.T., Evans, W.H., Flint, N. & Regoeczi, E. (1982) Nature 298, 398–400.CrossRefGoogle Scholar
  17. 17.
    Cox, R.A. & Arnstein, H.R.V. (1964) Biochem. J. 93, 33C–35C.Google Scholar
  18. 18.
    Evans, W.H., Debanne, M.T., Flint, N. & Regoeczi, E. (1983) Biochem. J. (submitted)Google Scholar
  19. 19.
    Van Renswoude, J., Bridges, K.R., Harford, J.B. & Klausner, R.D. (1983) Proc. Nat. Acad. Sci. 79, 6186–6190.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • W. H. Evans
    • 1
  • N. Flint
    • 1
  • N. Hadjiivanova
    • 1
  1. 1.National Institute for Medical ResearchMill Hill, LondonUK

Personalised recommendations