Subcellular Strategies for Tracing Receptor-Mediated Ligand Uptake Pathways in Liver

  • W. H. Evans
  • N. Flint
  • N. Hadjiivanova
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Receptor-mediated uptake by liver of asialoglycoproteins, polypeptide hormones and a β-adrenergic antagonist was followed by analytical centrifugation in sucrose gradients of tissue homogenates prepared at various intervals after organ perfusion. All ligands were transferred from the plasma membrane to a vesicular fraction of low density. Problems associated with the preparation of the complex low-density membrane fractions are described, and a preliminary description of their properties is provided.

Keywords

Sucrose Filtration Albumin Carbohydrate EDTA 

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • W. H. Evans
    • 1
  • N. Flint
    • 1
  • N. Hadjiivanova
    • 1
  1. 1.National Institute for Medical ResearchMill Hill, LondonUK

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