Advertisement

Epithelial Sodium Channels: Ligand Binding Techniques in Intact Tissues and Homogenates

  • A. W. Cuthbert
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)

Abstract

Sodium channels occur in the apical membranes of various epithelial cells, and are blocked by the pyrazine diuretic, amiloride. Using its N-benzyl derivative (labeled), [3H]benzamil, the choice of which is considered, binding studies have been performed under optimized conditions with intact epithelia and with homogenates. Related work, by current fluctuation analysis has been done elsewhere. Data are presented from experiments with frog skin epithelium, the avian coprodaeum, and rat kidney. The binding sites detected in the first two tissues have properties consistent with apical sodium channels, while those in kidney may be related to non-electrogenic sodium-proton exchange. Emphasis is placed throughout on physiological manipulations which affect both function and binding, and on the wide discrepancies in the amount of binding found in intact and homogenized tissues.

Keywords

Sodium Channel Sodium Transport Short Circuit Current Intact Tissue Frog Skin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bentley, P.J. (1979) in Amiloride and Epithelial Sodium Transport (Cuthbert, A.W., Fanelli, G. M. & Scriabine, A., eds.), Urban & Schwarzenburg, Baltimore/Munich, pp. 35–40.Google Scholar
  2. 2.
    Eigler, J., Kelter, J. & Renner, E. (1967) Klin. Woschr. 45, 737–741.CrossRefGoogle Scholar
  3. 3.
    Cuthbert, A.W. & Fanelli, G.M. (1978) Br. J. Pharmacol. 63, 139–149.CrossRefGoogle Scholar
  4. 4.
    Cuthbert, A.W. (1976) Mol. Pharmacol. 12, 945–957.Google Scholar
  5. 5.
    Cuthbert, A.W. & Edwardson, J.M. (1979) J. Pharm. Pharmacol. 31, 382–386.CrossRefGoogle Scholar
  6. 6.
    Cuthbert, A.W. (1973) J. Physiol. 228, 681–692.Google Scholar
  7. 7.
    Cuthbert, A.W. & Shum, W.K. (1974) Mol. Pharmacol. 10, 880–891.Google Scholar
  8. 8.
    Aceves, J., Cuthbert, A.W. & Edwardson, J.M. (1979) J. Physiol. 295, 477–490.Google Scholar
  9. 9.
    Aceves, J. & Cuthbert, A.W. (1979) J. Physiol. 295, 491–505.Google Scholar
  10. 10.
    Cuthbert, A.W. & Shum, W.K. (1976) J. Physiol. 260, 223–235.Google Scholar
  11. 11.
    Cuthbert, A.W. & Shum, W.K. (1977) Nature 266, 468–469.CrossRefGoogle Scholar
  12. 12.
    Cuthbert, A.W., Okpako, D. & Shum, W.K. (1974) Br. J. Pharmacol. 51, 128P-129P.Google Scholar
  13. 13.
    Lindemann, B. & Van Dreissche, W. (1977) Science 195, 292–294.CrossRefGoogle Scholar
  14. 14.
    Van Dreissche, W. & Lindemann, B. (1979) Nature 282, 519–520.CrossRefGoogle Scholar
  15. 15.
    Li, J.H.-Y., Palmer, L.G., Edeman, I.S. & Lindemann, B. (1982) J. Memo. Biol. 64, 77–89.CrossRefGoogle Scholar
  16. 16.
    Bindslev, N., Cuthbert, A.W., Edwardson, J.M., & Skadhauge, E. (1982) Pflüg. Arch. 392, 340–346.CrossRefGoogle Scholar
  17. 17.
    Cuthbert, A.W., Edwardson, J.M., Bindslev, N. & Skadhauge, E. (1982) Pflüg. Arch. 392, 347–351.CrossRefGoogle Scholar
  18. 18.
    Doyle, D.D., Wong, M., Tanaka, J. & Barr, L. (1982) Science 215, 1117–1119.CrossRefGoogle Scholar
  19. 19.
    Malysheva, M.K., Stefanov, A.V., Chagovetz, A.M. & Lishko, V.K. (1982) Biochim. Biophys. Acta 688, 246–250.CrossRefGoogle Scholar
  20. 20.
    Stoner, L.C., Burg, M.R. & Orloff, J. (1974) Am. J. Physiol. 227, 453–459.Google Scholar
  21. 21.
    O’Neil, R.G. & Boulpaep, E.L. (1979) J. Memb. Biol. 50, 365–387.CrossRefGoogle Scholar
  22. 22.
    Cuthbert, A.W. & Edwardson, J.M. (1981) Biochem. Pharmacol. 30, 1175–1183.CrossRefGoogle Scholar
  23. 23.
    Edwardson, J.M., Fanestil, D.D., Ellory, J.C. & Cuthbert, A.W. (1981) Biochem. Pharmacol. 30, 1185–1189.CrossRefGoogle Scholar
  24. 24.
    Kinsella, J.C. & Aronson, P.S. (1980) Am. J. Physiol. 238, F461–469.Google Scholar
  25. 25.
    Kinsella, J.C. & Aronson, P.S. (1981) Am. J. Physiol. 241, F374–379.Google Scholar
  26. 26.
    La Belle, E.F. & Lee, S.O. (1982) Biochemistry 21, 2693–2697.CrossRefGoogle Scholar
  27. 27.
    Cuthbert, A.W. & Spayne, J.A. (1983) Br. J. Pharmacol., in press.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • A. W. Cuthbert
    • 1
  1. 1.Department of PharmacologyUniversity of CambridgeCambridgeUK

Personalised recommendations