Molecular Genetics Applied to Receptor Analysis
A new dimension in receptor structure is now appearing, from the application of the powerful approach of molecular genetics to this field. If the cDNA’s coding for the receptor protein can be obtained by cloning technology, sequencing of the DNA is completely feasible and very rapid, and the amino acid sequences of the subunits can at once be deduced by means of the genetic code. In view of the vanishingly small amounts of protein that can be obtained when a brain receptor is purified by present methods (as surveyed in #A-9 above), and the difficulties of purifying such a receptor with all of its subunits present and intact, we can predict that the cDNA approach will become, instead, the route for determining the amino acid sequences of receptor subunits in general. Cloning of receptor genes will introduce, furthermore, many other new possibilities into receptor analysis, as I shall briefly consider here.
KeywordsAdenylate Cyclase Ternary Complex Xenopus Oocyte Target Size Receptor Analysis
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- 2.Barnard, E.A., Beeson, D., Bilbe, G., Brown, D.A., Constanti, A., Conti-Tronconi, B., Dolly, J.O., Dünn, S.M.J., Mehraban, F., Richards, B.M. & Smart, T.G. (1983) Cold Spr. Harb. Symp. 48, in press.Google Scholar
- 3.Sumikawa, K., Koughton, M., Entage, J.S., Richards, B.M. & Barnard, E.A. (1981) Nature 292, 862–864.Google Scholar
- 4.Smart, T.G., Constanti, A., Bilbe, G., Brown, D.A. & Barnard, E.A. (1983) Neurosci. Lett., in press.Google Scholar