Molecular Genetics Applied to Receptor Analysis

  • E. A. Barnard
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


A new dimension in receptor structure is now appearing, from the application of the powerful approach of molecular genetics to this field. If the cDNA’s coding for the receptor protein can be obtained by cloning technology, sequencing of the DNA is completely feasible and very rapid, and the amino acid sequences of the subunits can at once be deduced by means of the genetic code. In view of the vanishingly small amounts of protein that can be obtained when a brain receptor is purified by present methods (as surveyed in #A-9 above), and the difficulties of purifying such a receptor with all of its subunits present and intact, we can predict that the cDNA approach will become, instead, the route for determining the amino acid sequences of receptor subunits in general. Cloning of receptor genes will introduce, furthermore, many other new possibilities into receptor analysis, as I shall briefly consider here.


Adenylate Cyclase Ternary Complex Xenopus Oocyte Target Size Receptor Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Barnard, E.A., Miledi, R. & Sumikawa, K. (1982) Proc. R. Soc. B 215, 241–246.CrossRefGoogle Scholar
  2. 2.
    Barnard, E.A., Beeson, D., Bilbe, G., Brown, D.A., Constanti, A., Conti-Tronconi, B., Dolly, J.O., Dünn, S.M.J., Mehraban, F., Richards, B.M. & Smart, T.G. (1983) Cold Spr. Harb. Symp. 48, in press.Google Scholar
  3. 3.
    Sumikawa, K., Koughton, M., Entage, J.S., Richards, B.M. & Barnard, E.A. (1981) Nature 292, 862–864.Google Scholar
  4. 4.
    Smart, T.G., Constanti, A., Bilbe, G., Brown, D.A. & Barnard, E.A. (1983) Neurosci. Lett., in press.Google Scholar
  5. 5.
    Sumikawa, K., Houghton, M., Smith, J.C., Bell, L., Richards, B.M. & Barnard, E.A. (1982) Nucleic Acid Res. 10, 580–593.CrossRefGoogle Scholar
  6. 6.
    Raftery, M.A., Hunkapiller, M.W., Strader, C.D. & Hood, L.E. (1980 Science 208, 1454–1457.CrossRefGoogle Scholar
  7. 7.
    Nodu, M., Takahashi, H., Tanabe, T., Toyosato, M., Furukani, Y., Hirose, T., Asai, M., Inayami, S., Miyatu, T. & Numo, S. (1982) Nature 299, 793–797.CrossRefGoogle Scholar
  8. 8.
    Nodu, M., Takahashi, H., Tanabe, T., Toyosato, M., Kikyotani, S., Hirose, T., Asai, M., Takashima, H., Inayamu, S., Miyuku, T. (1983) Nature 301, 251–255.CrossRefGoogle Scholar
  9. 9.
    Claudio, T., Balliret, M., Patrick, J. & Heinemann, S. (1983) Proc. Nat. Acad. Sci. 80, 1111–1115.CrossRefGoogle Scholar
  10. 10.
    Comb, M., Seeburg, P.H., Adelman, J., Eider, L. & Herbert, E. (1982) Nature 295, 663–666.CrossRefGoogle Scholar
  11. 11.
    Nodu, M., Turutani, Y., Takahashi, H., Toyosato, M., Hirose, T., Inayama, S., Nakanishi, S. & Numa, S. (1982) Nature 295, 202–206.CrossRefGoogle Scholar
  12. 12.
    Nakanishi, S., Inone, A., Kita, T., Nakumura, M., Chang, A.C.Y., Cohen, S.N. & Numa, S. (1979) Nature 278, 423–427.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • E. A. Barnard
    • 1
  1. 1.Department of BiochemistryImperial College of Science and TechnologyLondonUK

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