Isolation of Purified Membranes and Membranous Cell Components for Receptor Studies

  • Gary R. Matyas
  • D. James Morré
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 13)


Subcellular fractionation of cells and tissues provides an important source of biochemical information concerning the structure, identification, characterization, biosynthesis, turnover and function of membrane-located receptors. Procedures are described that yield purified preparations of Golgi apparatus (G.a.), endoplasmic reticulum (e.r.J, plasma membrane (p.m.), mitochondria and nuclei from liver tissue; advantageously, the procedures can be carried out in a few hours using normal laboratory equipment.


Golgi Apparatus Homogenization Medium Fraction Purity Discontinuous Sucrose Gradient Swing Bucket Rotor 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Gary R. Matyas
    • 1
  • D. James Morré
    • 1
  1. 1.Department of Biological Sciences, Department of Medicinal Chemistry and Pharmacognosy, Purdue Cancer CenterPurdue UniversityWest LafayetteUSA

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