mRNA Distributions in Sea Urchin Embryos
We have developed techniques for localization of individual mRNA species in sections of sea urchin embryos using the hybridization of radioactively labeled RNA probes to tissue sections in situ. The method yields grain densities proportional to the concentration of target mRNAs and is sufficiently sensitive to allow the localization of most moderately abundant mRNAs. The major features of the method are briefly discussed, and several examples of its use are reviewed. We show that maternal histone mRNA is largely sequestered in pronuclei of unfertilized eggs and released at nuclear membrane breakdown of first cell division. We discuss several events in differentiation of cells of the dorsal ectoderm, including expression of a cell-type specific set of messenger RNAs.
KeywordsHistone Gene Histone mRNAs mRNA Distribution mRNA Strand Dorsal Ectoderm
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