Protein Synthesis Patterns During Early Amphibian Embryogenesis

  • Debbie Crise Meuler
  • George M. Malacinski


Changes in two-dimensional (2-D) gel protein synthesis patterns during the development of Ambystoma mexicanum and Xenopus laevis embryos were cataloged. Proteins which appear to be synthesized only during specific developmental stages were identified and a comparison of those results was made to similar types of analyses previously reported in the literature. It was concluded that neither species displayed changes in 2-D gel patterns during oocyte maturation. At fertilization, however, synthesis of some oogenic proteins ceased temporarily but resumed later in development. In Xenopus eggs the synthesis of several novel proteins was detected at fertilization. Later, during gastrulation, several additional novel proteins were also synthesized. During axolotl gastrulation, even more dramatic changes occurred. Ten percent of the proteins detected at earlier stages ceased synthesis at gastrulation. Furthermore, 10% of the proteins synthesized at gastrulation were novel. Some of them are specific to different tissues of the embryo. The actins and tubulins were also identified on the 2-D gels and their synthesis was monitored during axolotl development.


Xenopus Laevis Oocyte Maturation Xenopus Embryo Specific Developmental Stage Ambystoma Mexicanum 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Debbie Crise Meuler
    • 1
  • George M. Malacinski
    • 1
  1. 1.Program in Molecular, Cellular, and Developmental Biology, Department of BiologyIndiana UniversityBloomingtonUSA

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