Endothelial Cell Cultures: A New Model for the Study of Cerebral Vascular Endothelium

  • M. Spatz
  • I. Karnushina
  • I. Nagatsu
  • J. Bembry


So far, neither the studies of blood-brain barrier (BBB) nor of the isolated cerebral microvessels permitted investigation of the capillary endothelial function per se. Since the endothelium is the primary barrier between the blood and the brain and might be under neurohormonal regulation it became essential to develop a model system in which the endothelial properties and their response to vasoactive substances could be tested in the living state.


Adenylate Cyclase Adrenergic Receptor Vasoactive Substance Endothelial Cell Culture cAMP Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Axelrod J: Purification and properties of phenylethanolamine-N-methyltransferase. J Biol Chem 237: 1657–1660 (1962).Google Scholar
  2. 2.
    Edvinsson L, Sercombe R: Influence of pH and pCO2 on alphareceptor mediated contraction in brain vessels. Acta physiol Scan 97: 325–331 (1976).CrossRefGoogle Scholar
  3. 3.
    Hartman B: The innervation of cerebral blood vessels by central adrenergic neurons. In: Frontiers in catecholamine research, Usdin E, Snyder SH (Eds.) Pergamon Press, New York, 91–96 (1973).Google Scholar
  4. 4.
    Herbts TJ, Raichle ME, Ferrendelli JA: ß-adrenergic regulation of adenosine 3′, 5′-monophosphate concentration in brain micro-vessels. Science 204: 330–332 (1979).CrossRefGoogle Scholar
  5. 5.
    Karnushina IL, Spatz M, Bembry J: Cerebral endothelial cell culture. I. The presence of ß2 and ∝2-adrenergic receptors linked to adenylate cyclase activity. Life Sci 30: 849–858 (1982).CrossRefGoogle Scholar
  6. 6.
    Karnushina IL, Spatz M, Bembry J: Cerebral endothelial cell culture. II. Adenylate cyclase response to prostaglandins and their interaction with the adrenergic system. Life Sci (in press) (1982).Google Scholar
  7. 7.
    McGiff JC: Prostaglandins and blood pressure control. In: Biochemical regulation of blood pressure, Soffer RL (Ed.) John Wiley & Sons, New York, 359–389 (1981).Google Scholar
  8. 8.
    Nathanson JA: Cerebral microvessels contain a ß2-adrenergic receptor. Life Sci 26: 1793–1799 (1980).CrossRefGoogle Scholar
  9. 9.
    Palmer GC, Palmer SJ, Chronister RB: Cyclic nucleotide systems in the microcirculation of mammalian brains. In: The cerebral microvasculature, Eisenberg HM, Suddith RL (Eds.) Academic Press, New York, 147–162 (1980).CrossRefGoogle Scholar
  10. 10.
    Pickard JD: Role of prostaglandins and arachidonic acid derivatives in the coupling of cerebral blood flow to cerebral metablolism. J cerebral blood flow metabolism 1: 361–384 (1981).CrossRefGoogle Scholar
  11. 11.
    Preskorn SH, Hartman BK, Raichle ME, Swanson LW, Clark HB: Central adrenergic regulation of cerebral microvascular permeability and blood flow. In: The cerebral microvasculature, Eisenberg HM, Suddith RL (Eds.) Academic Press, 127–138.Google Scholar
  12. 12.
    Preskorn SH, Irwin GH, Simpson S, Friesen D, Rinne J, Jerkovich G: Medical therapies for mood disorders alter the blood-brain barrier. Science 213: 469–471 (1981).CrossRefGoogle Scholar
  13. 13.
    Sabol SL, Nirenberg M: Regulation of adenylate cyclase of neuroblastoma x glioma hybrid cells by ∝-adrenergic receptors. I. Inhibition of adenylate cyclase mediated by ∝ receptors. J Biol Chem 254: 1913–1920 (1979).Google Scholar
  14. 14.
    Spatz M, Bembry J, Dodson RF, Hervonen H, Murray MR: Endo-thelial cell cultures derived from isolated cerebral microvessels. Brain Res 191: 577–582 (1980).CrossRefGoogle Scholar
  15. 15.
    Spatz M, Nagatsu I, Maruki C, Yoshida M, Kondo Y, Bembry J: The presence of phenylethanolamine-N-methyltransferase in cerebral microvessel and endothelial cultures. Brain Res 240: 191–194 (1982).CrossRefGoogle Scholar
  16. 16.
    Van Calker D, Muller M, Hamprecht B: Adrenergic ∝ — and ß-receptors expressed by the same cell type in primary culture of perinatal mouse brain. J Neurochem 30: 713–718 (1978).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • M. Spatz
    • 1
  • I. Karnushina
    • 1
  • I. Nagatsu
    • 2
  • J. Bembry
    • 1
  1. 1.Laboratory of Neuropathology and Neuroanatomical Sciences, NINCDSNational Institutes of Health BethesdaUSA
  2. 2.Department of AnatomyFujita-Gakuen University, School of MedicineToyoakeJapan

Personalised recommendations