The Activities of Phospholipase Al, A2, Lysophospholipase and Acyl CoA: Lysophospholipid Acyltransferase in Ischemic Dog Brain

  • Yutaka Hirashima
  • Keiji Koshu
  • Kazuyo Kamiyama
  • Michiharu Nishijima
  • Shunro Endo
  • Akira Takaku


Biomembranes play an extremely important role in maintaining normal energy production in the cell and in the transport of essential molecules. The individual components of the cellular membrane itself are not stable, but rather undergo continuous turnover while maintaining homeostasis. Phospholipids are an important element in these membranes and particularly in the brain where membraneous structures are numerous, they constitute a large volume. The metabolism of brain phospholipid is consequently a topic of great interest.


Free Fatty Acid Membrane Phospholipid PLA2 Activity Occlusion Time Bilateral Carotid Artery Occlusion2 


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  1. 1.
    Allen RJL: The estimation of phosphorus: Biochem J, 34: 858–865 (1940).Google Scholar
  2. 2.
    Bazan NG Jr: Effect of ischemia and electroconvulsive shock on free fatty acid pool in the brain. Biochim Biophys Acta, 218: 1–10 (1970).CrossRefGoogle Scholar
  3. 3.
    Beyer-Mears A, Barnett A: dexamethasone preservation of motor function in phospholipase A2-induced caudate lesions. Exp Neurol 68: 240–248 (1980).CrossRefGoogle Scholar
  4. 4.
    Blomstrand R, Nakayama F: Neutron activation paper Chromatographic analysis of human cerebrospinal fluid. J Neurochem 8: 230–233 (1961).CrossRefGoogle Scholar
  5. 5.
    Broniszewska-Ardelt B, Dabrowiecki Z: Studies on the inhibition of brain phosphofructokinase by oleate and the determination of fatty acids in normal and hypoxic brain, J Neurochem 27: 585–587 (1976).CrossRefGoogle Scholar
  6. 6.
    Chan PH, Fishman RA: Brain edema: Induction in cortical slices by polyunsaturated fatty acids. Science, 201: 358–360 (1978).CrossRefGoogle Scholar
  7. 7.
    Cooper MF, Webster GR: On the phospholipase A2 activity of human cerebral cortex. J Neurochem, 19: 333–340 (1972).CrossRefGoogle Scholar
  8. 8.
    Corbin DR, Sun GY: Characterization of the enzymic transfer of arachidnoyl groups to 1-acylphosphoglycerides in mouse syn-aptosome fraction. J Neurochem 30: 77–82 (1978).CrossRefGoogle Scholar
  9. 9.
    Gullis RJ, Rowe CE: The stimulation of the phospholipase A2-acylation system of synaptic membranes of brain by cyclic nucleotides. Biochem J, 148: 567–581 (1975).Google Scholar
  10. 10.
    Gullis RJ, Rowe CE: The stimulation by transmitter substances and putative transmitter substances of the net activity of phospholipase A2 of synaptic membranes of cortex of guinea-pig brain. Biochem J, 148: 197–208 (1975).Google Scholar
  11. 11.
    Gullis RJ, Rowe CE: The stimulation by synaptic transmitters of the incorporation of oleate into the phospholipid of synaptic membranes. Biochem J, 148: 557–565 (1975).Google Scholar
  12. 12.
    Inoh S, Yoshida S, Sano K: Brain free fatty acids and phospho-lipids in experimental cerebral ischemia-role of post-ischemic lipid peroxidation. No To Shinkei, 32: 829–838 (1980) (Eng Abstr) (Jpn).Google Scholar
  13. 13.
    Kuwashima J, Fujitani B, Nakamura K, Kadokawa T, Yoshida K, Shimizu M: Biochemical changes in unilateral brain injury in the rat: A possible role of free fatty acid accumulation. Brain Res, 110: 547–557 (1976).CrossRefGoogle Scholar
  14. 14.
    Lands WEM: Metabolism of glycerolipid II. The enzymatic acyla-tion of lysolecithin. J Biol Chem, 235: 2233–2237 (1960).Google Scholar
  15. 15.
    Lunt GG, Rowe CE: The production of unesterified fatty acid in brain. Biochim Biophys Acta, 152: 681–693 (1968).CrossRefGoogle Scholar
  16. 16.
    Papadopulos NM, Cevallos W, Hess WC: Determination of phospho-lipids in spinal fluid and brain. Arch Neurol, 3: 677–682 (1960).CrossRefGoogle Scholar
  17. 17.
    Sun GY, Su KL, Der OM, Tang W: Enzymic regulation of arachido-nate metabolism in brain membraine phosphoglycerides. Lipid, 14: 229–235, (1978).CrossRefGoogle Scholar
  18. 18.
    Suzuki J, Yoshimoto T, Tanaka S, Sakamoto T: Production of various models of cerebral infarction in the dogs by means of occlusion of intracranial trunk arteries. Stroke, 11: 337–341 (1980).CrossRefGoogle Scholar
  19. 19.
    Thompson RHS, Niemiro R, Webster GR: The identification of lysolecithin in lipid extracts of brain. Biochim Biophys Acta, 43: 142–144 (1960).CrossRefGoogle Scholar
  20. 20.
    Webster GR, Thompson RHS: Observations of the presence of lysolecithin in nervous tissues. Biochim Biophys Acta, 63: 38–45 (1962).CrossRefGoogle Scholar
  21. 21.
    Webster GR, Alpern RJ: Studies of the acylation of lysolecithin by rat brain. Biochem J, 90: 35–42 (1964).Google Scholar
  22. 22.
    Webster GR, Thompson RHS: Accumulation of lysolecithin in rat brain on incubation in vitro Nature, 206: 296–297 (1965).Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Yutaka Hirashima
    • 1
  • Keiji Koshu
    • 1
  • Kazuyo Kamiyama
    • 1
  • Michiharu Nishijima
    • 1
  • Shunro Endo
    • 1
  • Akira Takaku
    • 1
  1. 1.Department of NeurosurgeryToyama Medical Pharmaceutical UniversityJapan

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