Cerebral Edema Formation and Blood-Brain Barrier Impairment by Intraventricular Collagenase Infusion

  • J. Gazendam
  • H. J. Houthoff
  • S. Huitema
  • K. G. Go


It has been well established that in some experimental models of brain edema formation a breakdown of the blood-brain barrier (BBB) to serum protein tracers may occur in cerebral endothelial cells that otherwise show no morphological signs of irreversible cell damage. as e.g. in early and intermediate phases of ischemic edema2,16,l2,3, in the marginal zone surrounding a cold injury9,1,6 and in the areas surrounding some primary and metastatic brain tumors. These observations suggest that one or more factors in the extracellular space (ECS) of the brain might be involved, which after being liberated by cellular damage may induce reversible changes in the endothelial cells (EC), whether directly or by involvement of the endothelial basement membrane (BM).


Brain Edema Vibratome Section Cerebral Microvessels Vascular Basement Membrane Protein Tracer 
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  1. 1.
    Baker RN, Cancilla PA, Pollock PS, Frommes SP: The movement of exogenous protein in experimental cerebral edema J. Neuropath exp. Neurol 30: 668–679 (1971).CrossRefGoogle Scholar
  2. 2.
    Fujimoto I, Walker JT Jr, Spatz M, Klatzo I: Pathophysiologic aspects of ischemic edema. In: Pappius HM, Feindel W (Eds.) Dynamics of brain edema. Springer, Berlin Heidelberg New York, 171–180 (1976).CrossRefGoogle Scholar
  3. 3.
    Go KG, Houthoff HJ, Huitema S, Spatz M: Protein tracer permeability of the blood-brain barrier after transient cerebral ischemia in gerbils. This symposium.Google Scholar
  4. 4.
    Godeau G, Robert AM: Mechanism of action of collagenase on the blood-brain barrier permeability. Increase of endothelial cell pinocytotic activity as shown with horseradish peroxidase as a tracer. Cell Biol Internat Report 3: 747–751 (1979).CrossRefGoogle Scholar
  5. 5.
    Houthoff HJ, Go KG: Endogenous versus exogenous protein tracer passage in blood-brain barrier damage. In: Cervos-Navarro J, Ferszt R (eds.) Brain Edema. Adv Neurol, 28. Raven Press, New York, 75–81 (1980).Google Scholar
  6. 6.
    Houthoff HJ, Go KG, Molenaar I: The permeability of the blood-brain barrier in acute hypertension. Comparison of an endogenous and exogenous protein tracer. Acta Neuropathol (Berl) (Suppl) 7: 13–16 (1981a).CrossRefGoogle Scholar
  7. 7.
    Houthoff HJ, Go KG, Huitema S: The permeability of cerebral capillary endothelium in cold injury. Comparison of an endogenous and exogenous protein tracer. In: Cervos-Navarro J, Fritschka E (Eds.). Cerebral microcirculation and metabolism. Adv neurol 29. Raven Press, New York, 331–336 (1981b).Google Scholar
  8. 8.
    Houthoff HJ, Go KG, Gerrits PO: The mechanisms of blood-brain barrier impairment by hyperosmolar perfusion. An electron cytochemical study comparing exogenous HRP and endogenous antibody to HRP as tracers. Acta Neuropathol (Berl) 56: 99–112 (1982).CrossRefGoogle Scholar
  9. 9.
    Klatzo I: Neuropathological aspects of brain edema. J Neuropathol Exp Neurol 24: 1–13 (1967).CrossRefGoogle Scholar
  10. 10.
    Liotta LA, Abe S, Robey PG, Martin GR: Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc Nat Acad Sci USA 76: 2268–2272 (1979).CrossRefGoogle Scholar
  11. 11.
    Nishimoto K, Wolman M, Spatz M, Klatzo I: Pathophysiological correlation in the blood-brain barrier damage to air embolism. In: Pathology of cerebral microcirculation 20. J Cervos Navarro (Ed), Raven Pres, New York 337–244 (1978).Google Scholar
  12. 12.
    Petito CK: Early and late mechanisms of increased vascular permeability following experimental cerebral infraction. J Neuro-pathl Exp Neurol 38: 222–234 (1979).CrossRefGoogle Scholar
  13. 13.
    Robert AM, Godeau G: Action of proteolytic and glycolytic enzymes on the permeability of the blood-brain barrier. Biomedicine 21: 36–39 (1974).Google Scholar
  14. 14.
    Robert AM, Godeau G, Miskulin M: Functional role of the macro-molecules of the intercellular matrix in the blood-brain barrier. In: Protides of the biological fluids, vol 22, 343–348 (1975).Google Scholar
  15. 15.
    Robert AM, Godeau G, Miskulin M, Moati F: Mechanism of action of collagenase on the permeability of the blood-brain barrier. Neurochem Res 2: 449–455 (1977).CrossRefGoogle Scholar
  16. 16.
    Westergaard E, Go KG, Klatzo I, Spatz M: Increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in Mongolian gerbils. Acta Neuropathol (Berl) 35: 307–325 (1976).Google Scholar
  17. 17.
    Woolley DE, Tethow LC, Mooney CJ, Evanson JM: Human collagenase and its extracellular inhibitors in relation to tumor invasive-ness. In: “Proteinases and Tumor Invasion” Sträuli P, Barrett AJ, Baici A. (Eds) Raven Press, New York, 97–115 (1980).Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • J. Gazendam
    • 1
  • H. J. Houthoff
    • 1
  • S. Huitema
    • 1
  • K. G. Go
    • 1
  1. 1.Departments of Neurosurgery and PathologyUniversity Hospital and University of GroningenGroningenThe Netherlands

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