Basic Molecular Events Underlying Transendothelial Transport in Brain Capillaries

  • Ferenc Joó
  • András Mihály
  • Péter Temesvári
  • Ernó Dux


Endothelial cells which are joined by tight junctions in the brain form a peculiar barrier restricting the movements of macro-molecules and many other solutes between blood and brain. So far. several enzymes including cholinesterases6,7, DOPA decarboxylase2 γ -glutamyl transpeptidase17. Na-K-activated adenosine triphospha-tase4,1, and other ATPases8,12 have been reported not only to be present in brain capillaries, but also to be involved in the regulation of blood-brain barrier permeability. Since the discovery of the importance of cyclic nucleotides in the mediation of the hormonal influences on different cellular functions, we have been trying to elucidate the possible role of cAMP and cGMP in the regulation of permeability of brain microvessels. Up to now, the following data have been collected:
  • dibutyryl (dibu) cAMP increases permeability and pinocytosis in brain capillaries9,

  • adenylate cyclase could be found in brain microvessels histochemically11,

  • histamine receptors — mainly of H2-type — are linked to the capillary adenylate cyclase as detected by biochemical measurements14,

  • the presence of guanylate cyclase was found in brain capillaries by means of histochemistry and biochemical measurements15.


Adenylate Cyclase Brain Edema Guanylate Cyclase Adenylate Cyclase Activity Adenosine Triphosphatase 
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  1. 1.
    Betz AL, Firth JA, Goldstein GW: Polarity of the blood-brain barrier: distribution of enzymes between the luminal and anti-luminal membranes of brain capillary endothelial cells. Brain Res. 192: 17–28 (1980).CrossRefGoogle Scholar
  2. 2.
    Bertler A, Falck B, Owman Ch, Rosengren E: The localization of monoaminergic blood-brain barrier mechanisms. Pharmacol. Rev. 18: 369–385 (1966).Google Scholar
  3. 3.
    Dux E, Joó F: Effects of histamine on the brain capillaries: fine structural and immunohistochemical studies after intra-carotic infusion. Exptl. Brain Res. (in press) (1982).Google Scholar
  4. 4.
    Eisenberg HM, Suddith RL: Cerebral vessels have the capacity to transport sodium and potassium. Science 206: 1083–1085 (1979).CrossRefGoogle Scholar
  5. 5.
    Hegstrand LR, Kanof PD, Greengard P: Histamine-sensitive adeny-late cyclase in mammalian brain. Nature, 260: 163–165 (1976).CrossRefGoogle Scholar
  6. 6.
    Joó F, Csillik B: Topographical correlation between the haema-toencephalic barrier and the cholinesterase activity of brain capillaries. Exptl. Brain Res., 1: 147–151 (1966).CrossRefGoogle Scholar
  7. 7.
    Joó F, Várkonyi T, Csillik B: Developmental alterations in the histochemical structures of brain capillaries: a histochemical contribution to the problem of the blood-brain barrier. Histo-chemie, 9: 140–148 (1967).Google Scholar
  8. 8.
    Joó F: The effect of the inhibition of adenosine triphosphatase activity on the fine structural organization of brain capillaries. Nature, 219: 1378–1379 (1968).CrossRefGoogle Scholar
  9. 9.
    Joó F: Effect of N6O6-dibutyryl cyclic 3′, 5′adenosine mono-phosphate on the pinocytosis of brain capillaries of mice. Experientia, 28: 1470–1471 (1972).CrossRefGoogle Scholar
  10. 10.
    Joó F, Karnushina I: A procedure for the isolation of capillaries from rat brain. Cytobios, 8: 41–48 (1973).Google Scholar
  11. 11.
    Joó F, Tóth I, Jancsó G: Brain adenylate cyclase: its common occurrence in the capillaries and astrocytes. Naturwissenschaften, 8: 397 (1975).CrossRefGoogle Scholar
  12. 12.
    Joó F: The role of adenosine triphosphatase in the maintenance of molecular organization of the basal lamina in the brain capillaries. Fron. Matrix Biol., Vol. 7 Robert L, (Ed.) pp 166–182 (1979).Google Scholar
  13. 13.
    Karnovsky MJ: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol., 35: 213–236 (1967).CrossRefGoogle Scholar
  14. 14.
    Karnushina IL, Palacios JM, Barbin G, Dux E, Joô F Schwartz JC: Studies on a capillary-rich fraction isolated from brain: histaminic components and characterization of the histamine receptors linked to adenylate cyclase. J. Neurochem., 34: 1201–1208 (1980).CrossRefGoogle Scholar
  15. 15.
    Karnushina I, Tóth I, Dux E, Joó F: Presence of the guanylate cyclase in brain capillaries: histochemical and biochemical evidence. Brain Res., 189: 588–592 (1980).CrossRefGoogle Scholar
  16. 16.
    Mihály A: Early ultrastructural changes in the cerebral cortex of albino rats subjected to 3-aminopyridine seizures. Experientia, 37: 870–871 (1981).CrossRefGoogle Scholar
  17. 17.
    Orlowski M, Sessa G, Green JP:/-Glutamyl transpeptidase in brain capillaries: possible site of a blood-brain barrier for amino acids. Science, 184: 66–68 (1974).CrossRefGoogle Scholar
  18. 18.
    Sternberger LA: Immunocytochemistry. Englewood Cliffs Prentice-Hall Inc. New Jersey (1974).Google Scholar
  19. 19.
    Wilmes F, Hossmann KA: A specific immunofluorescence technique for the demonstration of vasogenic brain edema in paraffin embedded material. Acta Neuropathol., 45: 47–51 (1979).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Ferenc Joó
    • 1
  • András Mihály
    • 2
  • Péter Temesvári
    • 3
  • Ernó Dux
    • 1
  1. 1.Laboratory of Molecular NeurobiologyInstitute of Biophysics, Biological Research CenterSzegedHungary
  2. 2.Department of AnatomyHistology and Embryology, University Medical SchoolSzegedHungary
  3. 3.Clinics of PediatricsUniversity Medical SchoolSzegedHungary

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