Correlated Electrophysiological and Biochemical Studies of Hippocampal Slices
The nervous system operates with impulses and transmissions that have time scales in the millisecond range, and yet is called upon to store information for periods of years. Evidently, the patterns of electrical activity that speed through brain circuitries, on some occasions, must modify the properties of the elements that transmit them. Understanding the nature of these modifications, the physiological forms they take, and the cellular chemistries that bring them into existence constitutes one of the major problems of neurobiology. Studies of the relatively simple nervous systems of invertebrates by Kandel and others have located synapses that are modified by experience (see Kandel, 1981, for a review). Comparable efforts on mammalian central nervous system (CNS), hampered as they are by the extraordinary complexity of the brain, are still at the stage of conclusively pinning down sites that show lasting traces of experience.
KeywordsHippocampal Slice Synaptic Membrane Perforant Path Phosphorylation Assay Hippocampal Membrane
Unable to display preview. Download preview PDF.
- Bliss, T. V. P. and Gardner-Medwin, A. T., 1973, Long-lasting potentiation of synaptic transmission in the dentate area of the unanaesthetized rabbit following stimulation of the perforant path, J. Physiol. (London) 232:357–374.Google Scholar
- Bliss, T. V. P. and Lomo, T., 1973, Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path, J. Physiol. (London) 232:331–356.Google Scholar
- Dunwiddie, T. V. and Lynch, G. S., 1978, Long-term potentiation and depression of synaptic responses in the rat hippocampus: Localization and frequency dependency, J. Physiol. (London) 276:353–367.Google Scholar
- Fagni, L., Baudry, M. and Lynch, G., 1983b, Classification and properties of acidic amino acid receptors in hippocampus. I. Electrophysiological studies of an apparent desensitization and interactions with drugs which block transmission, J. Neuroscience, 3:1538–1546.Google Scholar
- Kandel, E., 1981, Neuronal plasticity and the modification of behavior, in: Handbook of Physiology, Section I: The Nervous System (J. M. Brookhart, V. B. Mountcastle, E. R. Kandel, and S. R. Geiger, eds.), American Physiological Society, Baltimore, pp. 1137–1182.Google Scholar
- Lee, K., Schottler, F., Oliver, M., and Lynch, G., 1980, Brief bursts of high-frequency stimulation produce two types of structural change in rat hippocampus, J. Neurophy-siol. 44:247–258.Google Scholar
- Lee, K., Oliver, M., Schottler, F., and Lynch, G., 1981, Electron microscopic studies of brain slices: The effects of high frequency stimulation on dendritic ultrastructure, in: Electrical Activity in Isolated Mammalian CNS Preparations (G. Kerkut, ed.), Academic Press, New York, pp. 189–212.Google Scholar
- Lynch, G. and Baudry, M., Origins and Manifestations of Neuronal Plasticity in the hippocampus, in: Clinical Neurosciences (W. Willis, ed.), Churchill-Livingstone Publishers, New York, in press.Google Scholar
- Murachi, T., Hatanaka, M., Yasumoto, Y., Hakayata, N., and Tanaka, K., 1981a, A quantitative distribution study on calpain and calpastatin in rat tissues and cells, Biochem. Int. 2:651–656.Google Scholar
- Siman, R., Baudry, M. and Lynch, G., Purification from synaptosomal plasma membranes of calpain I, a thiol-protease activated by micromolar calcium concentrations, J. Neurochem., in press.Google Scholar