Advertisement

Brain Slices pp 285-296 | Cite as

Electrophysiological Study of the Neostriatum in Brain Slice Preparation

  • S. T. Kitai
  • H. Kita

Abstract

The neostriatum of mammalian species consists of the caudate and the putaman and is a major component of the basal ganglia (Carpenter, 1981). It is a relatively large nuclear mass and is easily dissected from the rest of the brain. In slice preparation, the neostriatum is severed from extrinsic connections. Neuronal responses recorded following electrical stimulation of the neostriatum in slice preparation therefore reflect only the activities of those neuronal elements left intact. These would include the soma, dendrites, and, so long as their course remains within the slice, axons of neostriatal neurons. Added to these are fibers of extrinsic origin and their terminals, isolated from their cell bodies, but remaining electrically excitable. The slice preparation therefore would be well suited to the study of local interactions among striatal neuronal elements and the action of monosynaptic afferents. The slice preparation enables the investigator to manipulate the extracellular milieu in a controlled manner. For this reason, it is also well suited for studying the action of putative neurotransmitters and other agents on the electrical activities of neurons and characterizing the relationships between these membrane activities and specific electrolytes.

Keywords

Recording Chamber Slice Preparation Medium Spiny Neuron Synaptic Potential Spike Potential 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Buchwald, N. A., Price, D. D., Vernon, L., and Hull, C. D., 1973, Caudate intracellular responses to thalamic and cortical inputs, Exp. Neurol. 38:311–323.PubMedCrossRefGoogle Scholar
  2. Carpenter, M. B., 1981, Anatomy of the corpus striatum and brain stem integrating systems, in: Handbook of Physiology, Section I: The Nervous System II Part 2 (V. B. Brooks, ed.), American Physiological Society, Bethesda, Maryland, pp. 947–995.Google Scholar
  3. Chang, H. T., Wilson, C. J., and Kitai, S. T., 1982, A Golgi study of rat neostriatal neurons: Light microscopic analysis, J. Comp. Neurol. 208:107–126.PubMedCrossRefGoogle Scholar
  4. DiFiglia, M., Pasik, P., and Pasik, T., 1976, A Golgi study of neuronal types in the neostriatum of monkeys, Brain Res. 114:245–256.PubMedCrossRefGoogle Scholar
  5. Fox, C. A., Andrade, A. N., Hillman, D. E., and Schwyn, R. C., 1971/72, The spiny neurons in the primate striatum: A Golgi and electron microscopic study, J. Hirnforsch. 13:181–201.PubMedGoogle Scholar
  6. Kemp, J. M. and Powell, T. P. S., 1971, The structure of the caudate nucleus in the cat: Light and electron microscopy, Phil. Trans. R. Soc. London, ser. B 262:383–401.CrossRefGoogle Scholar
  7. Kitai, S. T. and Bishop, G. A., 1981, Intracellular straining of neurons, in: Neuroanatomical Tract-tracing Methods (L. Heimer and M. J. Robards, eds.), Plenum Press, New York, pp. 263–277.CrossRefGoogle Scholar
  8. Kitai, S. T. and Wilson, C. J., 1982, Intracellular labeling of neurons in mammalian brain, in: Cytochemical Methods in Neuroanatomy (V. Chan-Palay and S. L. Palay, eds.), Alan R. Liss, Inc., New York, pp. 533–549.Google Scholar
  9. Kocsis, J. D. and Kitai, S. T., 1977, Dural excitatory inputs to caudate spiny neurons from substantia nigra stimulation, Brain Res. 138:271–283.PubMedCrossRefGoogle Scholar
  10. Lighthall, J. W., Park, M. R., and Kitai, S. T., 1981, Inhibition in slices of rat neostriatum, Brain Res. 212:182–187.PubMedCrossRefGoogle Scholar
  11. Misgeld, U. and Bak, I. J., 1979, Intrinsic excitation in the rat neostriatum mediated by acetylcholine, Neurosci. Lett. 12:277–282.PubMedCrossRefGoogle Scholar
  12. Park, M. R., Lighthall, J. W., and Kitai, S. T., 1980, Recurrent inhibition in the rat neostriatum, Brain Res. 194:359–369.PubMedCrossRefGoogle Scholar
  13. Preston, R. J., Bishop, G. A., and Kitai, S. T., 1980, Medium spiny neuron projection from the striatum: An intracellular horseradish peroxidase study, Brain Res. 183:253–263.PubMedCrossRefGoogle Scholar
  14. Somogyi, P. and Smith, A. D., 1979, Projection of neostriatal spiny neuron to the substantia nigra. Application of a combined Golgistaining and horseradish peroxidase transport procedure at both light and electron microscopic levels, Brain Res. 178:3–15.PubMedCrossRefGoogle Scholar
  15. Sugimori, M., Preston, R. J., and Kitai, S. T., 1978, Response properties and electrical constants of caudate nucleus neurons in the cat, J. Neurophysiol. 41:1662–1675.PubMedGoogle Scholar
  16. Wilson, C. J. and Groves, P. M., 1980, Fine structure and synaptic connections of the common spiny neuron of the rat neostriatum: A study employing intracellular injection of horseradish peroxidase, J. Comp. Neurol. 194:599–616.PubMedCrossRefGoogle Scholar
  17. Wilson, C. J., Chang, H. T., and Kitai, S. T., 1983, Disfacilitation and long-lasting inhibition of neostriatal neurons in the rat, Exp. Brain Res. 51:227–235.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • S. T. Kitai
    • 1
  • H. Kita
    • 1
  1. 1.Department of Anatomy, College of Medicine, Center for the Health SciencesThe University of TennesseeMemphisUSA

Personalised recommendations