Wheat α-Amylase Genes: Cloning of a Developmentally Regulated Gene Family
The achievement of successful molecular genetic engineering of plants as a widely used supplement to the conventional techniques of plant breeding is a distant goal for which advances in several areas of knowledge are required. Major areas of deficient knowledge may be broadly described as gene isolation, gene regulation and gene transfer. This chapter is intended to describe the isolation of a defined plant gene, the wheat α-amylase gene, as an illustration of a current approach to gene isolation from plant tissue. I also describe the use of the isolated gene as a probe of gene regulatory mechanisms.
KeywordsTranslation Product Distal Half Aleurone Cell Regulate Gene Family Rht3 Allele
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