The Application of Restriction Fragment Length Polymorphism to Plant Breeding
With the advent of the use of restriction endonucleases to map gene sequences, molecular geneticists have encountered widespread polymorphism for the location of restriction sites among individuals of a species. This observation is not surprising considering the number of supposedly neutral mutations encountered at the protean level in recent years. The genetic heterogeneity, called restriction fragment length polymorphism (RFLP), has been used to map linked genes (1) and is a potential tool in prenatal diagnosis (2). Considerable attention has been placed on the use of RFLPs in constructing a genetic map of the human genome to establish linkages with loci determining genetic diseases (3). The purpose of this paper is to point out the possible uses of this technique in agronomic improvement.
KeywordsRestriction Fragment Length Polymorphism Substitution Line Restriction Fragment Length Polymorphism Analysis Homoeologous Chromosome Restriction Fragment Length Polymorphism Marker
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