Advertisement

Transient Expression of Cloned Genes in Mammalian Cells

  • Julian Banerji
  • Walter Schaffner
Part of the Genetic Engineering book series (GEPM, volume 8)

Abstract

Diploid organisms do not lend themselves easily to the kind of classical genetic analysis that has led to the identification of many proteins, DNA sequences and pathways that play regulatory roles in haploid organisms. Instead, recent advances in our ability to clone individual genes, determine their primary structure and subsequently remodel them have led to a new way of asking questions about the regulation of gene expression in higher eukaryotes.

Keywords

Cold Spring Harbor Replication Origin Globin Gene Heat Shock Gene SV40 Enhancer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kressmann, A. and Birnstiel, M.L. (1980) in Transfer of Cell Constituents into Eukaryotic Cells (Celis, J.E., Graessmann, A. and Loyter, A., eds.), PP 383–407, NATO Advanced Study Institute Series A: Life Sciences, Vol.31.Google Scholar
  2. 2.
    Gurdon, J.B. and Melton, D.A. (1981) Annu. Rev. Gen. 15, 189–208.CrossRefGoogle Scholar
  3. 3.
    Graessmann, A., Graessmann, M. and Mueller, C. (1980) Methods Enzymol. 65, 816–825.PubMedCrossRefGoogle Scholar
  4. 4.
    Capecchi, M.R. (1980) Cell 22, 479–488.PubMedCrossRefGoogle Scholar
  5. 5.
    Graessmann, A., Graessmann, M. and Mueller, C. (1981) Advan. Cancer Res. 35, 111–149.CrossRefGoogle Scholar
  6. 6.
    Papahadjopoulos, D. and Straubinger, R.M. (1982) Methods Enzymol. (in press).Google Scholar
  7. 7.
    Furusawa, M., Nishimura, T., Yamaizumi, M. and Okada, Y. (1974) Nature 249, 449–450.PubMedCrossRefGoogle Scholar
  8. 8.
    Schaffner, W. (1980) Proc. Nat. Acad. Sci. U.S.A. 78, 2163–2167.CrossRefGoogle Scholar
  9. 9.
    Rassoulzadegan, M., Binetruy, B. and Cuzin, F. (1982) Nature 295, 257–259.PubMedCrossRefGoogle Scholar
  10. 10.
    Graham, F. and Van der Eb, A. (1973) Virology 52, 456–467.PubMedCrossRefGoogle Scholar
  11. 11.
    Pagano, J.S., McCutchan, H.G. and Vaheri, A. (1967) J. Virol. 1, 891–897.PubMedGoogle Scholar
  12. 12.
    Sompayrac, L.M. and Danna, K.J. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 7575–7578.CrossRefGoogle Scholar
  13. 13.
    Transfer of Cell Constituents into Eukaryotic Cells (1980) (Celis, J.E., Graessmann, A. and Loyter, A. eds.) NATO Advanced Study Institute Series A: Life Sciences, Vol.31.Google Scholar
  14. 14.
    Stunnenberg, H.G. and Birnstiel, M.L. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 6201–6204.CrossRefGoogle Scholar
  15. 15.
    Kucherlapati, R. (1982) in Advances in Cell Culture (Maramorosch, K., ed.) Vol.2, Academic Press, New York, NY (in press).Google Scholar
  16. 16.
    Schaffner, W., Topp, W. and Botchan, M. (1979) in Specific Eukaryotic Genes (Engberg, J., Klenow, H. and Leick, V. eds.) pp.191–203, Alfred Benzon Symposium 13, Copenhagen.Google Scholar
  17. 17.
    Mantei, N., Boll, W. and Weissmann, C. (1979) Nature 281, 40–46.PubMedCrossRefGoogle Scholar
  18. 18.
    Mulligan, R.C., Howard, B.H. and Berg, P. (1979) Nature 277, 108–114.PubMedCrossRefGoogle Scholar
  19. 19.
    Rigby, P.W.J. (1982) in Genetic Engineering 3 (Williamson, R., ed) pp.83–141, Academic Press, New York, NY.CrossRefGoogle Scholar
  20. 20.
    Weinberg, R.A. (1982) Cell 30, 3–4.PubMedCrossRefGoogle Scholar
  21. 21.
    Solnick, D. (1981) Cell 24, 135–143.PubMedCrossRefGoogle Scholar
  22. 22.
    Spaete, R.R. and Frenkel, N. (1982) Cell 30, 295–304.PubMedCrossRefGoogle Scholar
  23. 23.
    Panicali, D. and Paoletti, E. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 4927–4931.CrossRefGoogle Scholar
  24. 24.
    Eukaryotic Viral Vectors (1982) (Gluzman, Y., ed.), Cold Spring Harbor Press, Cold Spring Harbor, NY.Google Scholar
  25. 25.
    Hamer, D.H. and Leder, P. (1979) Cell 18, 1299–1302.PubMedCrossRefGoogle Scholar
  26. 26.
    Gruss, P., Efstratiadis, A., Karathanasis, S., Konig, S. and Khoury, G. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 943–947.CrossRefGoogle Scholar
  27. 27.
    Chu, G. and Sharp, P.A. (1981) Nature 289, 378–382.PubMedCrossRefGoogle Scholar
  28. 28.
    Subramani, S., Mulligan, R. and Berg, P. (1981) Mol. Cell. Biol. 1, 854–864.PubMedGoogle Scholar
  29. 29.
    Gething, M.-J. and Sambrook, J. (1982) Nature 300, 598–603.PubMedCrossRefGoogle Scholar
  30. 30.
    Hamer, D.H. (1980) in Genetic Engineering (Setlow, J.K. and Hollaender, A., eds), Vol.2, pp.83–101, Plenum Press, New York, NY.Google Scholar
  31. 31.
    Tooze, J. (1980) DNA Tumor Viruses, Cold Spring Harbor Press, Cold Spring Harbor, NY.Google Scholar
  32. 32.
    Banerji, J., Rusconi, S. and Schaffner, W. (1981) Cell 27, 299–308.PubMedCrossRefGoogle Scholar
  33. 33.
    Moreau, P., Hen, R., Wasylyk, B., Everett, R., Gaub, M.P. and Chambon, P. (1981) Nucl. Acids Res. 9, 6047–6068.PubMedCrossRefGoogle Scholar
  34. 34.
    Wasylyk, B., Wasylyk, K.C., Augereau, P. and Chambon, P. (1982) Cell (in press).Google Scholar
  35. 35.
    de Villiers, J. and Schaffner, W. (1981) Nucl. Acids Res. 9, 6251–6264.PubMedCrossRefGoogle Scholar
  36. 36.
    Lusky, M., Berg, L. and Botchan, M. (1982) in Eukaryotic Viral Vectors (Gluzman, Y., ed) pp.99–107, Cold Spring Harbor Press, Cold Spring Harbor, NY.Google Scholar
  37. 37.
    de Villiers, J., Olson, L., Banerji, J. and Schaffner, W. (1982) Cold Spring Harbor Symp.Quant.Biol. 47 (in press).Google Scholar
  38. 38.
    Conrad, S.E. and Botchan, M. (1982) Mol. Cell. Biol., 2, 949–965.PubMedGoogle Scholar
  39. 39.
    Gehring, C.A., Banerji, J., Serfling, E. and Schaffner, W. (unpublished erxperiments).Google Scholar
  40. 40.
    Sarver, N., Gruss, P., Law, M.F., Khoury, G. and Howley, P.M. (1981) Mol. Cell. Biol. 1, 486–496.PubMedGoogle Scholar
  41. 41.
    DiMaio, D., Treisman, R. and Maniatis, T. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 4030–4034.CrossRefGoogle Scholar
  42. 42.
    Binetruy, B., Rautmann, G., Meneguzzi, G., Breathnach, R. and Cuzin, F. (1982) in Eukaryotic Viral Vectors (Gluzman, Y., ed.), pp.89–92, Cold Spring Harbor Press, Cold Spring Harbor, NY.Google Scholar
  43. 43.
    Zinn, K., Mellon, P., Ptashne, M. and Maniatis, T. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 3897–4901.CrossRefGoogle Scholar
  44. 44.
    Gluzman, Y. (1981) Cell 23, 175–182.PubMedCrossRefGoogle Scholar
  45. 45.
    Lusky, M. and Botchan, M. (1981) Nature 293, 79–81.PubMedCrossRefGoogle Scholar
  46. 46.
    Meyers, R.M. and Tijan, R. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 6491–6495.CrossRefGoogle Scholar
  47. 47.
    Bergsma, D.J., Olive, D.M., Hartzell, S.W. and Subramanian, K.N. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 381–385.CrossRefGoogle Scholar
  48. 48.
    Gething, M.J. and Sambrook, J. (1981) Nature 293, 620–625.PubMedCrossRefGoogle Scholar
  49. 49.
    Mellon, P., Parker, V., Gluzman, Y. and Maniatis, T. (1981) Cell 27, 279–288.PubMedCrossRefGoogle Scholar
  50. 50.
    Tyndall, C., LaMantia, G., Thacker, C.M., Favaloro, J. and Kamen, R. (1981) Nucl. Acids Res. 9, 6231–6250.PubMedCrossRefGoogle Scholar
  51. 51.
    Grosveld, G.C., Shewmaker, C.K., Jat, P. and Flavell, R.A. (1981) Cell 25, 215–226.PubMedCrossRefGoogle Scholar
  52. 52.
    Grosveld, G.C., de Boer, E., Shewmaker, C.K. and Flavell, R.A. (1982) Nature 295, 120–126.PubMedCrossRefGoogle Scholar
  53. 53.
    Dierks, P., Wieringa, B., Marti, D., Reiser, J., van Ooyen, A., Meyer, F., Weber, H. and Weissmann, C. (1981) in ICN-UCLA Symposium on Molecular and Cellular Biology. XXXIII, (D.D. Brown and C.F. Fox, eds.) pp.347–366, Academic Press, New York, NY.Google Scholar
  54. 54.
    Grosveld, G.C., Rosenthal, A. and Flavell, R.A. (1982) Nucl. Acids Res. 10, 4951–4971.PubMedCrossRefGoogle Scholar
  55. 55.
    Dierks, P., van Ooyen, A., Cochran, M.D., Dobkin, C., Reiser, J. and Weissmann, C. (1982) Nature (submitted).Google Scholar
  56. 56.
    Picard, D. and Schaffner, W. (1983) Proc. Nat. Acad. Sci. U.S.A. 80 (in press). (submitted).Google Scholar
  57. 57.
    Busslinger, M., Moschonas, N. and Flavell, R.A. (1981) Cell 27, 289–298.PubMedCrossRefGoogle Scholar
  58. 58.
    Wieringa, B., Meyer, F., Reiser, J. and Weissmann, C. (1983) Nature (in press).Google Scholar
  59. 59.
    Treisman, R., Proudfoot, N.J., Shander, M. and Maniatis, T. (1982) Cell 29, 903–911.PubMedCrossRefGoogle Scholar
  60. 60.
    Pelham, H.R.B. (1982) Cell 30, 517–528.PubMedCrossRefGoogle Scholar
  61. 61.
    Treisman, R. and Maniatis, T. (personal communication).Google Scholar
  62. 62.
    Humphries, R.K., Ley, T., Turner, P., Moulton, A.D. and Nienhuis, A.W. (1982) Cell 30, 173–183.PubMedCrossRefGoogle Scholar
  63. 63.
    deVilliers, J., Olson, L., Tyndall, C. and Schaffner, W. (1982) Nucl. Acids Res. 10 (in press).Google Scholar
  64. 64.
    Laimins, L.A., Khoury, G., Gorman, C., Howard, B. and Gruss, P. (1982) Proc. Nat. Acad. Sci. U.S.A., 79, 6453–6457.CrossRefGoogle Scholar
  65. 65.
    Banerji, J., Gehring, C.A. and Schaffner, W. (unpublished experiments).Google Scholar
  66. 66.
    Contreras, R., Gheysen, D., Knowland, J., van de Voorde, A. and Fiers, W. (1982), Nature 300, 500–505.PubMedCrossRefGoogle Scholar
  67. 67.
    Gluzman, Y. (personal communication.Google Scholar
  68. 68.
    Gerster, T. and Schaffner, W. (manuscript in preparation).Google Scholar
  69. 69.
    Maniatis, T., Hardison, R.C., Lacy, E., Lauer, J., O’Connell, C., Quon, D., Sim, G.K. and Efstratiadis, A. (1978) Cell 15, 687–701.PubMedCrossRefGoogle Scholar
  70. 70.
    Dierks, P., van Ooyen, A., Mantei, N. and Weissmann, C. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 1411–1415.CrossRefGoogle Scholar
  71. 71.
    Gluzman, Y., Sambrook, J. and Frisque, R.J. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 3898–3902.CrossRefGoogle Scholar
  72. 72.
    Rio, D., Robbins, A., Myers, R. and Tijan, R. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 5706–5710.CrossRefGoogle Scholar
  73. 73.
    Weaver, R.F. and Weissmann, C. (1979) Nucl. Acids Res. 7, 1175–1193.PubMedCrossRefGoogle Scholar
  74. 74.
    Weber, H., Dierks, P., Meyer, F., van Ooyen, A., Dobkin, C., Abrescia, P., Kappeler, M., Meyhack, B., Zeltner, A., Mullen, E. and Weissmann, C. (1981) ICN-UCLA Symposium on Molecular and Cellular Biology XXXIII (D.D. Brown and C.F. Fox, eds.) pp.367–385, Academic Press, New York, NY.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Julian Banerji
    • 1
  • Walter Schaffner
    • 1
  1. 1.Institut für Molekularbiologie IIUniversität ZürichHönggerberg, ZürichSwitzerland

Personalised recommendations