Microcloning of Microdissected Chromosome Fragments

  • V. Pirrotta
  • H. Jackle
  • J. E. Edstrom
Part of the Genetic Engineering book series (GEPM, volume 8)


An average mammalian chromosome contains around 0.1 pg of DNA while an average Drosophila chromosome is about five times smaller. In dipteran insects, however, salivary gland chromosomes are highly polytenized, in the case of Drosophila containing up to 2000 copies of the euchromatic arms of the chromosomes (for giant mutants). These copies are arranged in parallel bundles of chromatin which, in ways not yet understood, undergo local condensation to give rise to a characteristic band-inter-band pattern visible in the phase microscope. Genetic mapping using visible rearrangements or deletions frequently allows the localization of Drosophila genes to within a single band-interband interval.


Chromosome Fragment Aqueous Droplet Salivary Gland Chromosome Dimethyl Dichlorosilane Saturated Phenol 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • V. Pirrotta
    • 1
  • H. Jackle
    • 1
  • J. E. Edstrom
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergW. Germany

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