Correlation Between a Mutant APRT Protein and Altered DNA in CHO Cells

  • Anne E. Simon
  • Milton W. Taylor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


The frequency of mutation to 2,6-diaminopurine resistance occurs at a much higher than expected frequency for a diploid autosomal locus. Spontaneous 2,6-diaminopurine resistant mutants have been reported to occur at frequencies of 3 x 10−6 in CHO cells1 and as high as 1 x 10−3 in mouse L-cells2. Such mutants appeared to arise in a single step; all were defective in the enzyme APRT and resistant to high concentrations (20–40 µg/ml) of 2,6-diaminopurine (DAP).


Dextran Sulfate Ethyl Methane Sulfonate Ethyl Methane Autosomal Recessive Mutant Adenine Phosphoribosyltransferase 
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  1. 1.
    M. W. Taylor, J. H. Pipkorn, M. K. Tokito, and R. 0. Pozzatti, Jr., Purine mutants of mammalian cell lines III: Control of purine biosynthesis in adenine phosphoribosyltransferase mutants of CHO cells. Somat. Cell Genet. 3: 195–206 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    J. A. Tischfield, J. J. Trill, U. I. Lee, K. Coy, and M. W. Taylor, Genetic instability at the adenine phosphoribosyltransferase locus in mouse L-cells. Mol. and Cell. Biol. 2: 250–257 (1982).Google Scholar
  3. 3.
    L. H. Thompson, S. Fong, and K. Brookman, Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells. Mutation Res. 74: 21–36 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    W. E. C. Bradley and D. Letovanec, A high-frequency non-random mutational event at the adenine phosphoribosyltransferase (APRT) locus of sib selected variants heterozygous for APRT. Somat. Cell Genet 8: 51–66 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    A. E. Simon, M. W. Taylor, W. E. C. Bradley, and L. H. Thompson, A model involving gene inactivation in the generation of autosomal recessive mutants in mammalian cells in culture. Mol. and Cell. Biol. (in press).Google Scholar
  6. 6.
    E. M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517 (1975).PubMedCrossRefGoogle Scholar
  7. 7.
    G. M. Wald, M. Stern, and G. Stark, Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxmethyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. (USA) 76: 3683–3687 (1979).CrossRefGoogle Scholar
  8. 8.
    I. Lowy, A. Pellicer, J. F. Jackson, G. Sim, S. Silverstein, and R. Axel, Isolation of transforming DNA: Cloning the hamster APRT gene. Cell 22: 817–823 (1980).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Anne E. Simon
    • 1
  • Milton W. Taylor
    • 1
  1. 1.Program in Genetics Department of BiologyIndiana UniversityBloomingtonUSA

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