Correlation Between a Mutant APRT Protein and Altered DNA in CHO Cells
The frequency of mutation to 2,6-diaminopurine resistance occurs at a much higher than expected frequency for a diploid autosomal locus. Spontaneous 2,6-diaminopurine resistant mutants have been reported to occur at frequencies of 3 x 10−6 in CHO cells1 and as high as 1 x 10−3 in mouse L-cells2. Such mutants appeared to arise in a single step; all were defective in the enzyme APRT and resistant to high concentrations (20–40 µg/ml) of 2,6-diaminopurine (DAP).
KeywordsDextran Sulfate Ethyl Methane Sulfonate Ethyl Methane Autosomal Recessive Mutant Adenine Phosphoribosyltransferase
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