Orotate Uptake and Metabolism by Human Erythrocytes
The uptake of the pyrimidine nucleotide precursor, orotate, by non-nucleated human erythrocytes is not immediately explicable, (1). During the course of an investigation into the origin of the increased erythrocyte nucleotides seen in hereditary ythrocyte pyrimidine 5′nucleotidase deficiency, rapid uptake of 14C orotate and its conversion to uridine nucleotides by normal red cells was observed (Figure 1(a) A). Moreover, free uridine was present within the cells and accumulated in significant quantities in the medium (Figure 2).
KeywordsNucleate Cell Ammonium Phosphate Perchloric Acid Extract Washed Erythrocyte Uridine Nucleotide
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