Substance Rates of Different Steps of Purine Metabolism in Normal and Preserved Red Blood Cells (RBC) Studied in Experiments Simulating In Vivo Conditions
In RBC the energy provided by glycolysis is transferred by ATP to energy requiring processes as ion pumping, chemical synthesis etc by means of ATP. That explains the use of this substance as a marker for cell viability after preservation of erythrocytes and also the addition of precursors — adenine, adenosine, inosine (for the ribose phosphate part) etc — to preservation media. The physiological role of RBC in the transport of purine compounds between the organs of the body has been discussed for years. In order to answer questions hidden within these fields of exploration more firm data describing the substance rates of the specific steps of purine metabolism in RBC are needed.
KeywordsPurine Nucleoside Purine Metabolism Substance Rate Adenylate Energy Charge Preservation Medium
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- 1.C.-H. de Verdier, A. Ericson, F. Niklasson and T. Groth, Erythrocyte metabolism of purines and purine nucleosides during storage and simulated physiological conditions, in “Red Cell Metabolism and Function,” G. J Brewer, ed., New York (1981).Google Scholar
- 3.Å. Ericson, F. Niklasson and C.-H. de Verdier, Systematic study of nucleotide analysis of human erythrocytes using anionic exchanges and HPLC. Clin Chim Acta. Submitted for publication.Google Scholar