Use of Adenine in Blood Banking

  • Grant R. Bartlett
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


The use of an acid-citrate-glucose mixture as a preservative/anticoagulant for the cold storage of human blood for purposes of transfusion developed out of research carried out during World War II in the United States and England. With this preservative it was possible to hold blood for three weeks with at least 70% post-transfusion survival of the red cells, longer storage leading to rapidly decreasing viability. The so-called ACD, and CPD (a slight variant), preservatives have been in use since the mid 1940’s until last year when supplementation with adenine has allowed extension of the storage outdating time from 3 to 5 weeks. The purpose of the present communication is to summarize key events leading to this use of adenine.1


Xanthine Oxidase Cold Storage Adenosine Deaminase Blood Banking Organic Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    G.R. Bartlett, in: “The Human Red Cell in Vitro,” T.J. Greenwalt and G.A. Jamieson, eds., Grune & Stratton, Inc., New York (1974) p. 5.Google Scholar
  2. 2.
    D.M. Donohue, C.A. Finch and B.W. Gabrio, J. Clin. Invest. 35: 562 (1956).PubMedCrossRefGoogle Scholar
  3. 3.
    F.M. Huennekens, E. Nurk and B.W. Cabrio, J. Biol. Chem. 221: 971 (1956).PubMedGoogle Scholar
  4. 4.
    B.W. Cabrio, C.A. Finch and F.M. Huennekens, Blood 11: 103 (1956).Google Scholar
  5. 5.
    R.D. Lange, W.H. Crosby, D.M. Donohue, et al, J. Clin. Invest. 37: 1485 (1958).PubMedCrossRefGoogle Scholar
  6. 6.
    K. Nakao, T. Wada, T. Kamiyama, et al, Nature 194: 877 (1962).PubMedCrossRefGoogle Scholar
  7. 7.
    A.W. Shafer and G.R. Bartlett, J. Clin. Invest. 41: 690 (1962).PubMedCrossRefGoogle Scholar
  8. 8.
    E.R. Simon, Blood 20: 485 (1962).PubMedGoogle Scholar
  9. 9.
    E.R. Simon, Transfusion 17: 317 (1977).PubMedCrossRefGoogle Scholar
  10. G.R. Bartlett, in: “Erythrocytes, Thrombocytes, Leukocytes,” E. Gerlach, K. Moser, E. Deutsch, et al, eds., Thieme, Stuttgart (1973) p. 139.Google Scholar
  11. 11.
    S.N. Swisher, Transfusion 17: 309 (1977).PubMedCrossRefGoogle Scholar
  12. 12.
    G.R. Bartlett, Transfusion 17: 351 (1977).PubMedCrossRefGoogle Scholar
  13. 13.
    G.R. Bartlett, Transfusion 17: 358 (1977).PubMedCrossRefGoogle Scholar
  14. 14.
    G.R. Bartlett, Transfusion 17: 367 (1977).PubMedCrossRefGoogle Scholar
  15. 15.
    W.L. Warner, Transfusion 17: 326 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Grant R. Bartlett
    • 1
  1. 1.Laboratory for Comparative BiochemistrySan DiegoUSA

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