Isotope Studies of Uric Acid Metabolism During Dietary Purine Administration

  • Werner Löffler
  • Wolfgang Gröbner
  • Ramiro Medina
  • Nepomuk Zöllner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


Uric acid is excreted by the kidneys and intestinal tract, while other routes are negligible. By addition of purines to a purine-free or low purine diet changes in uric acid turnover and excretion occur, which cannot be investigated by metabolic balance methods as that part of the uric acid turnover secreted into the gut is completely destroyed by the intestinal flora (Sorensen, 1960). We therefore studied uric acid pool size and turnover in normal subjects both during a purine-free diet and during purine supplementation by stable isotope methods.


Uric Acid Turnover Study Stable Isotope Method Uric Acid Clearance Uric Acid Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J.D. Benedict, P.H. Forsham, D. Stetten, The metabolism of uric acid in the normal and gouty human studied with the aid of isotopic uric acid, J. Biol. Chem. 181, 183 (1949)PubMedGoogle Scholar
  2. 2.
    L.A. Johnson, B.T. Emmerson, Isolation of crystalline uric acid from urine, for urate pool and turnover measurements, Clin. Chim. Acta 41, 389 (1972)CrossRefGoogle Scholar
  3. 3.
    W. Löffler, Harnsäurepool and Harnsäureumsatz, in: “Hyperurikämie und Gicht”, N. Zöllner, ed.,Springer, Berlin - Heidelberg - New York (1980)Google Scholar
  4. 4.
    J. Ross, E. Martin, A rapid procedure for preparing gas samples for nitrogen-15 determination, The Analyst 95, 817 (1970)CrossRefGoogle Scholar
  5. 5.
    L.B. Sorensen, The elimination of uric acid in man studied by means of C 14-labbelled uric acid, Scand. J. Clin. Lab.Invest. 12, Supp1. 54 (1960)Google Scholar
  6. 6.
    F.J. Winkler, H-L. Schmidt, Einsatzmöglichkeiten der 13 C-Isotopen-Massenspektrometrie in der Lebensmitteluntersuchung, Z. Lebensm. Unters.Forsch. 171, 85 (1980)CrossRefGoogle Scholar
  7. 7.
    N. Zöllner, Eine einfache Modifikation der enzyma- tischen Harns.urebestimmung, Z. klin. Chem. klin. Biochem. 1, 178 (1963)Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Werner Löffler
    • 1
  • Wolfgang Gröbner
    • 1
  • Ramiro Medina
    • 2
  • Nepomuk Zöllner
    • 1
  1. 1.Medizinische PoliklinikUniversität MünchenGermany
  2. 2.Lehrstuhl für Allgemeine Chemie und BiochemieTechniscen Universität München Freising-WeihenstephanGermany

Personalised recommendations