Optimized Micro-Measurement of H.PRTase Activity on Fibroblasts and Hair Follicles
Detection of heterozygotes which are carriers of Lesh and Nyhan’s syndrome, poses a difficult problem, which has up to the present, not been solved in a very satisfactory way in current practice. This had led us, for genetic counseling, to reconsider in detail the determination methods of H.PRTase activity in cultured cells and in hair follicles.
KeywordsGenetic Counseling Hair Follicle Incubation Medium DEAE Cellulose Newborn Calf Serum
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