Adenosine Deaminase, Purine Nucleoside Phosphorylase and 5′-Nucleotidase Activities in Infectious Mononucleosis

  • Johannes Mejer
  • Per Nygaard
  • Jørgen Cohn
  • Ole Gadeberg
  • Viggo Faber
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


The present study was undertaken to study 3 selected purine pathway enzymes in infectious mononucleosis (IM). The enzymes concerned are adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and 5′-nucleotidase (5′-N). The enzymes were selected since the absence of these enzymes have been associated with clinically defective lymphoid function (1), and because abnormalities in the activities of ADA and PNP have been observed in leukemia (2,3) In 1974 Quagliata et al. (4) claimed that 5′-N may also be usefull in the biochemical characterization of subgroups of patients with lymphoid leukemia. In the early phase of IM they found a decrease of 5′-N.


Acute Myeloid Leukemia Chronic Lymphocytic Leukemia Chronic Myeloid Leukemia Adenosine Deaminase Infectious Mononucleosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    I. H. Fox, Metabolic basis for disorders of purine nucleotide degradation. Metabolism. 30: 616 (1981).PubMedCrossRefGoogle Scholar
  2. 2.
    D. G. Poplack, J. Blatt, and G. Reaman, Purine pathway enzyme abnormalities in acute lymphoblastic leukemia. Cancer Res., 41: 4821 (1981).PubMedGoogle Scholar
  3. 3.
    J. Mejer and P. Nygaard, Adenosine deaminase and purine nucleoside phosphorylase levels in acute myeloblastic leukemia cells. Relationship to diagnosis and clinical course. Leukemia Res., 3: 211 (1979).CrossRefGoogle Scholar
  4. 4.
    F. Quagliata, D. Faig, M. Conly, and R. Silber, Studies on the lymphocyte 5′-nucleotidase in chronic lymphocytic leukemia, infectious mononucleosis, normal subpopulations, and phytohemagglutinin-stimulated cells. Cancer Res., 34: 3197 (1974).PubMedGoogle Scholar
  5. 5.
    A. Boyum, Separation of blood leukocytes, granulocytes and lymphocytes. Tissue Antigens. 4: 269 (1974).PubMedCrossRefGoogle Scholar
  6. 6.
    J. Mejer and P. Nygaard, Ageing and activities of purine metabolizing enzymes in leukocytes. In Inborn Errors of Immunity and Phagocytosis (F. Güttler, J. W. T. Seakins and R. A. Harkness, Eds.) p. 181 MTP Press.Google Scholar
  7. 7.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. L. Randall, Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265 (1951).PubMedGoogle Scholar
  8. 8.
    M. Papamichail, P. J. Sheldon, and E. J. Holborow, T and B-cell subpopulations in infectious mononucleosis. Clin. exp. Immunol., 18: 1 (1974).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Johannes Mejer
    • 1
  • Per Nygaard
    • 2
  • Jørgen Cohn
    • 3
  • Ole Gadeberg
    • 3
  • Viggo Faber
    • 3
  1. 1.Blood Serology DepartmentBispebjerg HospitalDenmark
  2. 2.University Institute of Biological Chemistry BDenmark
  3. 3.Department of Infectious Diseases M, RigshospitaletCopenhagenDenmark

Personalised recommendations